Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma

dc.creatorAlmeida, Tarcísio Vila Verde Santana de
dc.creatorFernandes, Jamille Souza
dc.creatorLopes, Diego Mota
dc.creatorAndrade, Lorena Santana
dc.creatorOliveira, Sérgio Costa
dc.creatorCarvalho Filho, Edgar Marcelino
dc.creatorAraujo, Maria Ilma
dc.creatorCruz, Álvaro A
dc.creatorCardoso, Luciana Santos
dc.date2017-03-14T14:18:50Z
dc.date2017-03-14T14:18:50Z
dc.date2017
dc.date.accessioned2023-09-26T22:21:33Z
dc.date.available2023-09-26T22:21:33Z
dc.identifierALMEIDA, T. V. V. S. et al. Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma. Acta Tropica, v. 166, p. 268–279, 2017.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18034
dc.identifier10.1016/j.actatropica.2016.12.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878297
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAsthma is a chronic disease characterized by airway inflammation, obstruction and hyperresponsiveness. Severe asthma affects a small proportion of subjects but results in most of the morbidity, costs and mortality associated with the disease. Studies have suggested that Schistosoma mansoni infection reduces the severity of asthma and prevent atopy. Objective: We evaluated the ability of S. mansoni antigens, Sm29 and Sm29TSP-2 to modulate lymphocyteactivation status in response to the allergen of the mite Dermatophagoides pteronyssinus (Der p1) in cellcultures of individuals with asthma.Methods: Thirty four patients were enrolled in this study: seventeen patients with severe asthma (SAgroup), seventeen patients with mild asthma (MA group) and six controls with no asthma. Peripheralblood mononuclear cells (PBMC) were obtained and stimulated with Sm29 and Sm29TSP-2 in the pres-ence or absence of Der p1. The expression of surface markers and cytokines on lymphocytes was evaluatedby flow cytometry and the levels of IL-10 in the culture supernatant were determined by ELISA.Results: The addition of Sm29 and Sm29TSP-2 antigens to PBMC cultures from both groups of subjectswith asthma stimulated with Der p1 reduced the frequency of CD4+CD25lowcells whereas and increasedfrequency of CD4+CD25highpopulation was observed compared to unstimulated cultures. Moreover, cul-tures stimulated with Sm29TSP-2 showed a reduction in the frequency of T cells expressing CD69, IFN- ,TNF and TGF- in the MA group and an increase in the frequency of CD4+TSLPR+T cells in the SA group.The addition of Sm29 to the cultures reduced the frequency of CD4+CD69+and CD4+IL-5+T cells in all asth-matic groups, and reduced the frequency of CD4+T cells expressing IL-13 in the MA group. The culturesstimulated with Sm29 and Sm29TSP-2 showed an increase in the level of IL-10 in the supernatants.Conclusion: These results suggest that the addition of Sm29 and Sm29TSP-2 to the cells cultures fromsubjects with asthma reduced cell activation markers and altered the cytokine production pattern in away that can potentialy control the inflammatory response associated with asthma
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectAsma
dc.subjectAntígenos de Schistosoma mansoni
dc.subjectLinfócitos
dc.subjectSm29
dc.subjectSm29TSP-2a
dc.subjectAsthma
dc.subjectSchistosoma mansoni antigens
dc.subjectLymphocytes
dc.subjectSm29
dc.subjectSm29TSP-2a
dc.titleSchistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma
dc.typeArticle


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