Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus

dc.creatorVieira, Debora Ferreira Barreto
dc.creatorJácome, Fernanda Cunha
dc.creatorSilva, Marcos Alexandre Nunes da
dc.creatorCaldas, Gabriela Cardoso
dc.creatorFilippis, Ana Maria Bispo de
dc.creatorSequeira, Patrícia Carvalho de
dc.creatorSouza, Elen Mello de
dc.creatorAndrade, Audrien Alves
dc.creatorManso, Pedro Paulo de Abreu
dc.creatorTrindade, Gisela Freitas
dc.creatorLima, Sheila Maria Barbosa
dc.creatorBarth, Ortrud Monika
dc.date2017-11-14T13:12:48Z
dc.date2017-11-14T13:12:48Z
dc.date2017
dc.date.accessioned2023-09-26T22:21:22Z
dc.date.available2023-09-26T22:21:22Z
dc.identifierVIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23162
dc.identifier10.1371/journal.pone.0184397
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878264
dc.descriptionZika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectZika Virus
dc.subjectBrasil
dc.subjectCélulas Vero
dc.subjectPesquisa
dc.subjectZika virus
dc.subjectBrazil
dc.subjectVero cells
dc.subjectC6/36 cells
dc.subjectstructural investigation
dc.titleStructural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
dc.typeArticle


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