dc.creatorRosse, Izinara da Cruz
dc.creatorSteinberg, Raphael da Silva
dc.creatorCoimbra, Roney Santos
dc.creatorPeixoto, Maria Gabriela Campolina Diniz
dc.creatorVerneque, Rui Silva
dc.creatorMachado, Marco Antonio
dc.creatorFonseca, Cleusa Graça
dc.creatorCarvalho, Maria Raquel Santos
dc.date2015-07-21T16:11:27Z
dc.date2015-07-21T16:11:27Z
dc.date2014
dc.date.accessioned2023-09-26T22:20:36Z
dc.date.available2023-09-26T22:20:36Z
dc.identifierROSSE, Izinara da Cruz et al. Novel SNPs and INDEL polymorphisms in the 3′UTR of DGAT1 gene: in silico analyses and a possible association. Molecular Biology Reports, vol. 41, n.7 p. 4555-4563, 2014
dc.identifier0301-4851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11396
dc.identifier10.1007/s11033-014-3326-z.
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878102
dc.descriptionDiacylglycerol–O–acyltransferase (DGAT1) gene encodes the rate-limiting enzyme of triglyceride synthesis. A polymorphism in this gene, DGAT1 K232A, has been associated with milk production and composition in taurine breeds. However, this polymorphism is not a good tool for ascertaining the effects of this QTL in Bos indicus (Zebu), since the frequency of the DGAT1 232A allele is too low in these breeds. We sequenced the 3′-untranslated region of DGAT1 gene in a sample of bulls of the breeds Guzerá (Bos indicus) and Holstein (Bos taurus) and, using in silico analysis, we searched for genetic variation, evolutionary conservation, regulatory elements, and possible substitution effects. Six single nucleotide (SNPs) and one insertion-deletion (INDEL) polymorphisms were found in the Guzerá bulls. Additionally, we developed a preliminary association study, using this INDEL polymorphism as a genetic marker. A significant association was detected (P ≤ 0.05) between the INDEL (DGAT1 3′UTR INDEL) and the breeding values (BV) for protein, fat, and milk yields over a 305-day lactation period. The DGAT1 3′ UTR INDEL genotype I/I (I, for insertion) was associated with lower BVs (−38.77 kg for milk, −1.86 kg for fat, and −1.48 kg for protein yields), when compared to the genotype I/D (D, for deletion). I/D genotype was lower D/D genotype (−34.98 kg milk, −1.73 kg fat, and −1.09 kg protein yields). This study reports the first polymorphism of DGAT1 3′UTR in the Guzerá breed, as well as its association with BV for milk protein, fat, and milk yields.
dc.formatapplication/pdf
dc.languageeng
dc.publisherReidel
dc.rightsrestricted access
dc.subjectGenetic association study
dc.subjectBos indicus
dc.subjectDGAT1
dc.subject30Untranslated region
dc.titleNovel SNPs and INDEL polymorphisms in the 3′UTR of DGAT1 gene: in silico analyses and a possible association
dc.typeArticle


Este ítem pertenece a la siguiente institución