Functional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon

dc.creatorSousa, Leijiane F.
dc.creatorPortes Junior, José A.
dc.creatorNicolau, Carolina A.
dc.creatorBernardoni, Juliana L.
dc.creatorNishiyama Jr, Milton Y.
dc.creatorAmazonas, Diana R.
dc.creatorSousa, Luciana A. Freitas de
dc.creatorMourão, Rosa H. V.
dc.creatorChalkidis, Hipócrates M.
dc.creatorValente, Richard H.
dc.creatorSilva, Ana M. Moura da
dc.date2017-11-14T10:20:11Z
dc.date2017-11-14T10:20:11Z
dc.date2017
dc.date.accessioned2023-09-26T22:20:17Z
dc.date.available2023-09-26T22:20:17Z
dc.identifierSOUSA, Leijiane F. et al. Functional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon. Journal of Proteomics, v.159, p.32–46, Mar.2017.
dc.identifier1874-3919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23142
dc.identifier10.1016/j.jprot.2017.03.003
dc.identifier1876-7737
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8878031
dc.descriptionVenom variability is commonly reported for venomous snakes including Bothrops atrox. Here, we compared the composition of venoms from B. atrox snakes collected at Amazonian conserved habitats (terra-firme upland forest and várzea) and human modified areas (pasture and degraded areas). Venom samples were submitted to shotgun proteomic analysis as a whole or compared after fractionation by reversed-phase chromatography. Whole venom proteomes revealed a similar composition among the venoms with predominance of SVMPs, CTLs, and SVSPs and intermediate amounts of PLA2s and LAAOs. However, when distribution of particular isoforms was analyzed by either method, the venom from várzea snakes showed a decrease in hemorrhagic SVMPs and an increase in SVSPs, and procoagulant SVMPs and PLA2s. These differences were validated by experimental approaches including both enzymatic and in vivo assays, and indicated restrictions in respect to antivenom efficacy to variable components. Thus, proteomic analysis at the isoform level combined to in silico prediction of functional properties may indicate venom biological activity. These results also suggest that the prevalence of functionally distinct isoforms contributes to the variability of the venoms and could reflect the adaptation of B. atrox to distinct prey communities in different Amazon habitats.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectSerpentes
dc.subjectVenenos de Serpentes
dc.subjectJararaca
dc.subjectProteômica funcional
dc.subjectSnale
dc.subjectVenom
dc.subjectVariability
dc.subjectBothrops atrox
dc.subjectFunctional proteomics
dc.subjectAntivenom
dc.subjectVariabilidade
dc.titleFunctional proteomic analyses of Bothrops atrox venom reveals phenotypes associated with habitat variation in the Amazon
dc.typeArticle


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