Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

dc.creatorMendes, Rita Gabriela Pedrosa Ribeiro
dc.creatorGusmão, Michélia Antônia do Nascimento
dc.creatorMaia, Ana Carolina Ribeiro Gomes
dc.creatorDetoni, Michelle de Lima
dc.creatorPorcino, Gabriane Nascimento
dc.creatorSoares, Thais Vieira
dc.creatorJuliano, Maria Aparecida
dc.creatorJuliano, Luiz
dc.creatorCoelho, Paulo Marcos Zech
dc.creatorLenzi, Henrique Leonel
dc.creatorPinto, Priscila de Faria
dc.creatorVasconcelos, Eveline Gomes
dc.date2014-11-24T18:01:13Z
dc.date2014-11-24T18:01:13Z
dc.date2011
dc.date.accessioned2023-09-26T22:20:06Z
dc.date.available2023-09-26T22:20:06Z
dc.identifierMENDES, Rita Gabriela Pedrosa Ribeiro et al. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 7, p. 808-813, 2011.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8953
dc.identifier10.1590/S0074-02762011000700005
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877993
dc.descriptionA peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectSchistosoma mansoni
dc.subjectNTPDase
dc.subjectpotato apyrase
dc.subjectpeptide
dc.titleImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro¬lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg
dc.typeArticle


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