Brazilian essential oil of Cymbopogon martini (Roxb.) Will. Watson (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging

dc.creatorOliveira, Carlos Rocha
dc.creatorBella, Leonardo Mendes
dc.creatorDuarte, Marília Cristina
dc.creatorVieira, Rodolfo de Paula
dc.date2022-01-16T20:44:35Z
dc.date2022-01-16T20:44:35Z
dc.date2021
dc.date.accessioned2023-09-26T22:20:00Z
dc.date.available2023-09-26T22:20:00Z
dc.identifierOLIVEIRA, Carlos Rocha et al. Brazilian essential oil of Cymbopogon martini (Roxb.) Will. Watson (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging. Revista Fitos, v. 15, n. 4, p. 444-455, Dec. 2021.
dc.identifier1808-9569
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50772
dc.identifier10.32712/2446-4775.2021.1127
dc.identifier2446-4775
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877972
dc.descriptionThe study evaluated the effects of essential oil from Cymbopogon martini (Roxb.) Will. Watson (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblasts. Samples were collected in Monte Verde, Minas Gerais, Brazil and analyzed by gas chromatography with mass spectrometry. The fibroblasts were cultured in a monolayer using Iscove's medium and stimulated by LPS (1 μg/mL) and incubated for 24 h at 37°C. The cytotoxicity was evaluated by MTT assay and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1 (CCL2), and MIP-1-α (CCL3) were evaluated. The effect of CMEO on the expression of mRNA and the secretion of enzymes in fibroblasts were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10 μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid than control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α than control cells. The study demonstrated the effects of CMEO on the modulation of mediators related to inflammation and decreasing the mRNA and secretion levels of metalloproteinases, revealing to be a promising candidate for anti-aging effects and wound healing treatments.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Centro de Inovação em Biodiversidade e Saúde
dc.rightsopen access
dc.subjectCymbopogon martini
dc.subjectAnti-inflammatory
dc.subjectAnti-aging
dc.subjectHyaluronic acid
dc.subjectCollagen
dc.titleBrazilian essential oil of Cymbopogon martini (Roxb.) Will. Watson (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging
dc.typeArticle


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