Perinatal analyses of Zika- and dengue virus-specific neutralizing antibodies: A microcephaly case-control study in an area of high dengue endemicity in Brazil

dc.creatorCastanha, Priscila M. S.
dc.creatorSouza, Wayner V.
dc.creatorBraga, Cynthia
dc.creatorAraújo, Thália Velho Barreto de
dc.creatorXimenes, Ricardo A. A.
dc.creatorAlbuquerque, Maria de Fátima P. M.
dc.creatorMontarroyos, Ulisses R.
dc.creatorMiranda-Filho, Demócrito B.
dc.creatorCordeiro, Marli T.
dc.creatorDhalia, Rafael
dc.creatorMarques, Ernesto T. A.
dc.creatorRodrigues, Laura C.
dc.creatorMartelli, Celina M. T.
dc.date2019-10-01T14:52:03Z
dc.date2019-10-01T14:52:03Z
dc.date2019
dc.date.accessioned2023-09-26T22:19:59Z
dc.date.available2023-09-26T22:19:59Z
dc.identifierCASTANHA, Priscila M. S. et al. Perinatal analyses of Zika- and dengue virus-specific neutralizing antibodies: A microcephaly case-control study in an area of high dengue endemicity in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 3, p. 1-17, 2019.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36040
dc.identifier10.1371/journal.pntd.0007246
dc.identifier1935-2735
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877967
dc.descriptionLaboratory confirmation of Zika virus (ZIKV) infection during pregnancy is challenging due to cross-reactivity with dengue virus (DENV) and limited knowledge about the kinetics of anti-Zika antibody responses during pregnancy. We described ZIKV and DENV serological markers and the maternal-fetal transfer of antibodies among mothers and neonates after the ZIKV microcephaly outbreak in Northeast Brazil (2016). We included 89 microcephaly cases and 173 neonate controls at time of birth and their mothers. Microcephaly cases were defined as newborns with a particular head circumference (2 SD below the mean). Two controls without microcephaly were matched by the expected date of delivery and area of residence. We tested maternal serum for recent (ZIKV genome, IgM and IgG3 anti-NS1) and previous (ZIKV and DENV neutralizing antibodies [NAbs]) markers of infection. Multiple markers of recent or previous ZIKV and DENV infection in mothers were analyzed using principal component analysis (PCA). At delivery, 5.6% of microcephaly case mothers and 1.7% of control mothers were positive for ZIKV IgM. Positivity for ZIKV IgG3 anti-NS1 was 8.0% for case mothers and 3.5% for control mothers. ZIKV NAbs was slightly higher among mothers of cases (69.6%) than that of mothers of controls (57.2%; p = 0.054). DENV exposure was detected in 85.8% of all mothers. PCA discriminated two distinct components related to recent or previous ZIKV infection and DENV exposure. ZIKV NAbs were higher in newborns than in their corresponding mothers (p<0.001). We detected a high frequency of ZIKV exposure among mothers after the first wave of the ZIKV outbreak in Northeast Brazil. However, we found low sensitivity of the serological markers to recent infection (IgM and IgG3 anti-NS1) in perinatal samples of mothers of microcephaly cases. Since the neutralization test cannot precisely determine the time of infection, testing for ZIKV immune status should be performed as early as possible and throughout pregnancy to monitor acute Zika infection in endemic areas.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectZika
dc.subjectDengue
dc.subjectMicrocephaly
dc.subjectInfection
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectAnticorpos Neutralizantes / sangue
dc.subjectAnticorpos Antivirais / sangue
dc.subjectBrasil / epidemiologia
dc.subjectEstudos de Casos e Controles
dc.subjectDengue / diagnóstico
dc.subjectDengue / epidemiologia
dc.subjectVírus da Dengue / imunologia
dc.subjectFeminino
dc.subjectHumanos
dc.subjectImunoglobulina G / sangue
dc.subjectImunoglobulina M / sangue
dc.subjectRecém-Nascido
dc.subjectMasculino
dc.subjectMicrocefalia / epidemiologia
dc.subjectMicrocefalia / etiologia
dc.subjectGravidez
dc.subjectComplicações Infecciosas na Gravidez / diagnóstico
dc.subjectComplicações Infecciosas na Gravidez / epidemiologia
dc.subjectAdulto Jovem
dc.subjectZika virus / imunologia
dc.subjectInfecção por Zika virus / complicações
dc.subjectInfecção por Zika virus / diagnóstico
dc.subjectInfecção por Zika virus / epidemiologia
dc.titlePerinatal analyses of Zika- and dengue virus-specific neutralizing antibodies: A microcephaly case-control study in an area of high dengue endemicity in Brazil
dc.typeArticle


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