Iron modulates ecto-phosphohydrolase activities in pathogenic trichomonads

dc.creatorJesus, José Batista de
dc.creatorFerreira, Marcelo Alves
dc.creatorCuervo, Patrícia
dc.creatorBritto, Constança
dc.creatorSilva Filho, Fernando Costa e
dc.creatorFernandes, José Roberto Meyer
dc.date2022-02-16T14:18:39Z
dc.date2022-02-16T14:18:39Z
dc.date2006
dc.date.accessioned2023-09-26T22:19:35Z
dc.date.available2023-09-26T22:19:35Z
dc.identifierJESUS, José Batista de et al. Iron modulates ecto-phosphohydrolase activities in pathogenic trichomonads. Parasitology International, v. 55, p. 284-290, 2006.
dc.identifier1383-5769
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51239
dc.identifier10.1016/j.parint.2006.08.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877876
dc.descriptionThe presence of iron in the extracellular medium is essential for both in vivo and in vitro survival of pathogenic microorganisms, including Trichomonas vaginalis and Tritrichomonas foetus. In these parasites, iron is directly involved in the proliferation, protein expression and activation of critical enzymes. The purpose of this study was to investigate the role of iron in ecto-ATPase, ecto-phophatase and secreted phosphatase activities of these trichomonads. We observed that trichomonads grown in iron-depleted medium exhibited a remarkable decrease in both ecto-ATPase and ecto-phosphatase activities, when compared to those cultivated under control conditions (iron-rich medium). Furthermore, parasites grown in iron-depleted medium restored their enzyme activities when they were re-inoculated into fresh iron-rich medium. We demonstrated that modulation of ecto-phosphohydrolase activities is due neither to enzyme–iron nor to substrate–iron complex formation, since iron addition directly to the medium where the enzymatic reactions occurred did not alter their activities. Previously, we had reported that a fresh clinical isolate of T. vaginalis was much more cytotoxic to epithelial cell monolayers than a long-term cultured one. In this study we witnessed that the fresh isolate of T. vaginalis presented higher activities to all herein investigated enzymes than the long-term cultured one. Altogether, our data clearly point out that iron has a pivotal role in the expression of phosphohydrolases in both trichomonads.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectTrichomonas vaginalis
dc.subjectTritrichomonas foetus
dc.subjectFerro
dc.subjectEcto-ATPase
dc.subjectEcto-fosfatase
dc.subjectTrichomonas vaginalis
dc.subjectTritrichomonas foetus
dc.subjectIron
dc.subjectEcto-ATPase
dc.subjectEcto-phosphatase
dc.titleIron modulates ecto-phosphohydrolase activities in pathogenic trichomonads
dc.typeArticle


Este ítem pertenece a la siguiente institución