Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)

dc.creatorAlencar, Jeronimo
dc.creatorGuimarães, Anthony Érico
dc.creatorMello, Rubens Pinto
dc.creatorLopes, Catarina Macedo
dc.creatorDégallier, Nicolas
dc.creatorSantos-Mallet, Jacenir R.
dc.date2019-07-18T13:54:04Z
dc.date2019-07-18T13:54:04Z
dc.date2003
dc.date.accessioned2023-09-26T22:18:58Z
dc.date.available2023-09-26T22:18:58Z
dc.identifierALENCAR, Jeronimo et al. Scanning electron microscopy of the eggs of Haemagogus leucocelaenus (Diptera: Culicidae). Revista de Saúde Pública, v. 37, n. 5, p. 1-7, Oct. 2003.
dc.identifier0034-8910
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/34233
dc.identifier10.1590/S0034-89102003000500017
dc.identifier1518-8787
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877735
dc.descriptionOBJECTIVE: To observe morphological details of the eggs of Haemagogus (Conopostegus) leucocelaenus, seen for the first time by scanning electron microscopy (SEM), with morphometric analysis of the main structures. METHODS: Eggs of Hg. leucocelaenus were obtained from females captured in the Biological Reserve of Tinguá, State of Rio de Janeiro. Some of the eggs were kept for hatching and others underwent processing for scanning electron microscopy studies. Three eggs were submitted to morphometric analysis. The material was fixed in 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide, both in 0.1M, pH 7.2 sodium cacodylate buffer, then dehydrated in ethanol and dried using the critical point method. This was then set up on metallic supports, covered with gold and observed using the Jeol 5310 scanning electron microscope. Measurements were made with the aid of the Semafore analysis software coupled to the electron microscope. RESULTS: The eggs presented elliptical outlines of approximately 574 mm in length and 169 mm in width, with an egg index (l/w ratio) of 3.39 mm. The exochorion was extremely regular and had ornamentation that was usually hexagonal but sometimes pentagonal. Tubercles were observed on the chorionic cells, symmetrically arranged in relation to the longitudinal axis. Inside the cells, there were smaller, individualized tubercles, some arranged peripherally and others grouped to a greater or lesser extent in the center. The surface of the chorionic reticulum did not present rugosity. The micropylar apparatus was formed by a prominent and continuous collar of 8.32 mm in thickness, with a slightly irregular surface. The micropylar disk was very evident, and was continuous with the collar. The micropyle was seen at the center of this disk, measuring 1.6 mm and with a micropylar apparatus of 27.3 mm in diameter. CONCLUSIONS: The ornamentation of the exochorion presents differences in relation to the tubercles of chorionic cells and the external chorionic reticulum between the eggs of Hg. Leucocelaenus, in comparison with the eggs of Hg. janthinomys and Hg. equinus, and also in relation to those of Aedes aegypti, Ae. albopictus and Ae bahamensis. In various aspects, the eggs of Hg. leucocelaenus have more resemblance to those of Hg. Equinus than those of Hg. janthinomys, with greater differences presented in relation to the eggs of Hg. spegazzinii and Hg. lucifer.
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidade de São Paulo. Faculdade de Saúde Pública
dc.rightsopen access
dc.subjectDiptera, ultraestrutura
dc.subjectUltraestrutura
dc.subjectCulicidae, ultraestrutura
dc.subjectOvos
dc.subjectHaemagogus
dc.subjectMicroscopia Eletrônica de Varredura
dc.subjectDiptera, ultrastructure
dc.subjectUltrastructure
dc.subjectCulicidae, ultrastructure
dc.subjectEggs
dc.subjectScanning electron microscopy
dc.subjectHaemagogus
dc.titleScanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
dc.typeArticle


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