Ion channels in volume regulation of clonal kidney cells

dc.creatorSilva, M. B. da
dc.creatorCosta, V. M. A.
dc.creatorPereira, V. R. A.
dc.creatorAlbertim, G. J. B. de
dc.creatorMelo, E. B. B. de
dc.creatorBezerra, D. P.
dc.creatorSilva, R. P. da
dc.creatorRodrigues, C. G.
dc.creatorCarneiro, C. M. M.
dc.creatorYuldasheva, L. N.
dc.creatorKrasilnikov, O. V.
dc.date2018-08-14T11:31:43Z
dc.date2018-08-14T11:31:43Z
dc.date2010
dc.date.accessioned2023-09-26T22:18:09Z
dc.date.available2023-09-26T22:18:09Z
dc.identifierDA SILVA, M. B. et al. Ion channels in volume regulation of clonal kidney cells. Cell Proliferation, v. 43, n. 6, p. 529–541, dez. 2010.
dc.identifier1365-2184
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28110
dc.identifier10.1111/j.1365-2184.2010.00702.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877545
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico
dc.descriptionObjectives:  Clonal kidney cells (Vero cells) are extensively utilized in the manufacture of biological preparations for disease diagnostics and therapeutics and also in preparation of vaccines. In all cells, regulation of volume is an essential function coupled to a variety of physiological processes and is a topic of interest. The objective here was to investigate involvement of ion channels in the process of volume regulation of Vero cells. Methods:  Involvement of ion channels in cell volume regulation was studied using video‐microscopy and flow cytometry. Pharmacologically unaltered cells of different sizes, which are presumably at different phases of the cell cycle, were used. Results:  Ion transport inhibitors altered all phases of regulatory volume decrease (RVD) of Vero cells, rate of initial cell swelling, Vmax and volume recovery. Effects were dependent on type of inhibitor and on cell size (cell cycle phase). Participation of aquaporins in RVD was suggested. Inhibitors decelerated growth, arresting Vero cells at the G0/G1 phase boundary. Electrophysiological study confirmed presence of volume‐activated Cl− channels and K+ channels in plasmatic membranes of the cells. Conclusion:  Vero cells of all sizes maintained the ability to recover from osmotic swelling. Activity of ion channels was one of the key factors that controlled volume regulation and proliferation of the cells.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subject4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / farmacologia
dc.subjectAnimais
dc.subjectCiclo Celular / efeitos de drogas
dc.subjectProliferação Celular / efeitos de drogas
dc.subjectTamanho da Célula / efeitos de drogas
dc.subjectCélulas Cultivadas
dc.subjectCercopithecus aethiops
dc.subjectCitometria de Fluxo
dc.subjectGliburide / farmacologia
dc.subjectCanais Ion / antagonistas e inibidores
dc.subjectCanais Ion / metabolismo
dc.subjectRim / citologia
dc.subjectRim / metabolismo
dc.subjectMicroscopia
dc.subjectNitrobenzoatos / farmacologia
dc.subjectTetraetilamónio / farmacologia
dc.subjectCélulas Vero
dc.titleIon channels in volume regulation of clonal kidney cells
dc.typeArticle


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