Higher cross-subtype IFN-γ ELISpot responses to Gag and Nef peptides in Brazilian HIV-1 subtype B- and F1- than in C-infected subjects

dc.creatorCôrtes, Fernanda Heloise
dc.creatorBello, Gonzalo
dc.creatorVorsatz, Carla
dc.creatorPilotto, José Henrique
dc.creatorGuimarães, Monick Lindenmeyer
dc.creatorGrinsztejn, Beatriz
dc.creatorVeloso, Valdiléa G.
dc.creatorPinto, Aguinaldo Roberto
dc.creatorMorgado, Mariza Gonçalves
dc.date2015-07-13T12:46:49Z
dc.date2015-07-13T12:46:49Z
dc.date2013
dc.date.accessioned2023-09-26T22:17:27Z
dc.date.available2023-09-26T22:17:27Z
dc.identifierCÔRTES, Fernanda Heloise et al. Higher cross-subtype IFN-γ ELISpot responses to Gag and Nef peptides in Brazilian HIV-1 subtype B- and F1- than in C-infected subjects. Vaccine, v. 31, n. 7, p. 1106–1112, Feb. 2013.
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11222
dc.identifier10.1016/j.vaccine.2012.12.023
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877387
dc.descriptionHIV-1 diversity has been considered a huge challenge for the HIV-1 vaccine development. To overcome it, immunogens based on centralized sequences, as consensus, have been tested. In Brazil, the co-circulation of three subtypes offers a suitable scenario to test T cell cross-subtype responses to consensus sequences. Furthermore, we included peptides based on closest viral isolates (CVI) from each subtype analyzed to compare with T cell responses detected against the consensus sequences. The study included 32 subjects infected with HIV-1 subtype B (n = 13),C (n = 11), and F1 (n = 8). Gag and Nef-specific T cell responses were evaluated by IFN- -ELISpot assay. Peptides based on CVI sequences were similar to consensus in both reducing genetic distance and detecting T cell responses. A high cross-subtype response between B and F1 in both regions was observed in HIV-1 subtype B and F1-infected subjects. We also found no significant difference in responses to subtype B and C consensus peptides among subtype B-infected subjects. In contrast, the magnitude of T cell responses to consensus C peptides in the Gag region was higher than to consensus B peptides among HIV-1 subtype C-infected subjects. Regarding Nef, subtype Cinfected subjects showed higher values to consensus C than to consensus F1 peptides. Moreover, subtype F1-infected subjects presented lower responses to subtype C peptides than to subtype F1 and B. A similar level of responses was detected with group M based peptides in subtype B and F1 infected subjects. However, among subtype C infected subjects, this set of peptides detected lower levels of response than consensus C. Overall, the level of cross-subtype response between subtypes B and F1 was higher than between subtype C and B or C and F1. Our data suggests that the barrier of genetic diversity in HIV-1 group M for vaccine design may be dependent on the subtypes involved.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectT-cell response
dc.subjectGag
dc.subjectNef
dc.subjectConsensus versus isolate sequences
dc.subjectCross-reactivity
dc.subjectHIV-1 subtypes
dc.subjectGenes gag
dc.subjectHIV-1
dc.titleHigher cross-subtype IFN-γ ELISpot responses to Gag and Nef peptides in Brazilian HIV-1 subtype B- and F1- than in C-infected subjects
dc.typeArticle


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