A Reassessment of the in vitro total protein content determination (TPC) with SIRC and 3T3 cells for the evaluation of the ocular irritation potential of shampoos: comparison with the in vivo Draize rabbit test

dc.creatorCosta, Rodrigo Netto
dc.creatorAbreu, Clarice Lima do Canto
dc.creatorPresgrave, Rosaura de Farias
dc.creatorAlves, Eloisa Nunes
dc.creatorPresgrave, Octavio Augusto França
dc.creatorDelgado, Isabella Fernandes
dc.date2015-12-10T11:18:09Z
dc.date2015-12-10T11:18:09Z
dc.date2011
dc.date.accessioned2023-09-26T22:17:19Z
dc.date.available2023-09-26T22:17:19Z
dc.identifierCOSTA, R. N. et al. A Reassessment of the in vitro total protein content determination (TPC) with SIRC and 3T3 cells for the evaluation of the ocular irritation potential of shampoos: comparison with the in vivo Draize rabbit test. Braz. Arch. Biol. Technol., Curitiba, v. 54, n. 6, p. 1135-1146, nov./dez. 2011.
dc.identifier1516-8913
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12370
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132011000600008
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877357
dc.descriptionThe aim of this work was to determine the correlation between results obtained from the Draize test and from the Total Protein Content Determination (TPC) to assess the ocular irritancy potential of 20 shampoos. For TPC, two established cell lines (SIRC and 3T3) were used. The concentration that induced 50% inhibition relative to controls (IC50) was calculated for each product. Among shampoos tested with SIRC, only one had a false positive result. However, for the 3T3, three false-negative results were found. Pearson coefficient related to the in vivo value of maximum average score (MAS) was -0.58 (p=0.007) with SIRC and -0.73 (p=0.007) with 3T3. These results showed that the TPC assay was capable to predict the ocular irritant potential of shampoos, and therefore was a promissory tool to be used as a preliminary assay for the detection of irritant products and to be part of a battery of screening tests to minimize the animal use in the Draize Test.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto de Tecnologia do Paraná
dc.rightsopen access
dc.subjectTeste Draize
dc.subjectIrritação Ocular
dc.subjectDeterminação de Proteínas Totais
dc.subjectShampoos
dc.subjectCélulas SIRC
dc.subjectCélulas 3T3
dc.subjectDraize Test
dc.subjectOcular Irritation
dc.subjectTPC
dc.subjectShampoos
dc.subjectSIRC
dc.subject3T3
dc.subjectTestes de Irritação da Pele
dc.subjectAnimais de Laboratório
dc.subjectAlternativas aos Testes com Animais
dc.subjectTensoativos
dc.subjectTestes Imunológicos de Citotoxicidade
dc.subjectCélulas 3T3
dc.subjectCosméticos
dc.titleA Reassessment of the in vitro total protein content determination (TPC) with SIRC and 3T3 cells for the evaluation of the ocular irritation potential of shampoos: comparison with the in vivo Draize rabbit test
dc.typeArticle


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