Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal

dc.creatorCorrêa, Alex Pauvolid
dc.creatorMorales, Maria Alejandra
dc.creatorLevis, Silvana
dc.creatorFigueiredo, Luis Tadeu Moraes
dc.creatorLima, Dinair Couto
dc.creatorCampos, Zilca
dc.creatorNogueira, Marcia Furlan
dc.creatorSilva, Edson Elias da
dc.creatorNogueira, Rita Maria Ribeiro
dc.creatorSchatzmayr, Hermann Gonçalves
dc.date2017-01-03T16:24:04Z
dc.date2017-01-03T16:24:04Z
dc.date2011
dc.date.accessioned2023-09-26T22:16:16Z
dc.date.available2023-09-26T22:16:16Z
dc.identifierCORRÊA, Alex Pauvolid; et al. Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal. Mem Inst Oswaldo Cruz, v.106, n.4, p.467-474, June 2011.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16681
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877113
dc.descriptionDespite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of ≥ four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectJacarés e Crocodilos
dc.subjectVírus do Nilo Ocidental
dc.subjectPantanal
dc.subjectBrasil
dc.subjectEquinos
dc.subjectNeutralização
dc.subjectWest Nile virus
dc.subjectPantanal
dc.subjectneutralisation
dc.subjectequines
dc.subjectcaimans
dc.subjectBrazil
dc.titleNeutralising antibodies for West Nile virus in horses from Brazilian Pantanal
dc.typeArticle


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