In vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection

dc.creatorAranda-Souza, Mary Ângela
dc.creatorLorena, Virginia Maria Barros de
dc.creatorCorreia, Maria Tereza dos Santos
dc.creatorFigueiredo, Regina Célia Bressan Queiroz de
dc.date2019-05-24T15:23:00Z
dc.date2019-05-24T15:23:00Z
dc.date2018
dc.date.accessioned2023-09-26T22:16:11Z
dc.date.available2023-09-26T22:16:11Z
dc.identifierARANDA-SOUZA, Mary Ângela et al. In vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection. International Journal of Biological Macromolecules, v. 120, p. 431-439, Dec. 2018.
dc.identifier1879-0003
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33213
dc.identifier10.1016/j.ijbiomac.2018.08.064
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877096
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e Fundação de Amparo à Pesquisa do Estado de Pernambuco ( APQ / FACEPE 0.584-2,13 / 15 ). Aranda-Souza é doutoranda apoiada pela Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) em cooperação com a FIOCRUZ em uma bolsa de estudos Brasil sem Miséria.
dc.descriptionLeishmania amazonensis and Leishmania braziliensis are the main causative agents of American Tegumentary Leishmaniasis (ATL) in Brazil. As intracellular parasites, the infection by Leishmania species is dependent on the host immune response and the immunotherapy could be promissory for the development of new strategies to combat ATL. In this work we investigated the leishmanicidal potential of a galactose-binding lectin from the snake venom of Bothrops leucurus (BLL) during the infection with L. amazonensis and L. braziliensis. BLL inhibited the promastigote growth and viability of both species in a mechanism dependent on galactose and calcium. The treatment with BLL also decreases the survival of intracellular parasites for both species and induced profound ultrastructural changes on amastigotes without apparent damage to the host cells. The analysis of the cytokine profile revealed that BLL induced an increase in the proinflammatory cytokines IL-6 and TNF-α by infected macrophages in both species, but differed in relation to IL-1β and IL-10 response. Future works using in vitro and in vivo models are necessary to support the use of these lectins as biotechnological tool in immunological studies.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectLectin
dc.subjectLeishmania
dc.subjectImmunomodulation
dc.subjectAnimais
dc.subjectBothrops
dc.subjectBrasil
dc.subjectGalectinas / química
dc.subjectGalectinas / farmacologia
dc.subjectHumanos
dc.subjectLeishmania braziliensis / efeitos de drogas
dc.subjectLeishmania braziliensis / patogenicidade
dc.subjectLeishmaniose cutânea / quimioterapia
dc.subjectLeishmaniose cutânea / parasitologia
dc.subjectMacrófagos / efeitos de drogas
dc.subjectRatos
dc.subjectVenenos de cobra / química
dc.subjectVenenos de cobra / farmacologia
dc.titleIn vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection
dc.typeArticle


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