Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.

dc.creatorVasconcelos, Juliana Fraga
dc.creatorSouza, Bruno Solano de Freitas
dc.creatorLins, Thayse Fernanda da Silva
dc.creatorGarcia, Letícia Maria da Silva
dc.creatorKaneto, Carla Martins
dc.creatorSampaio, Geraldo Pedral
dc.creatorAlcantara, Adriano Costa de
dc.creatorMeira, Cássio Santana
dc.creatorMacambira, Simone Garcia
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorSoares, Milena Botelho Pereira
dc.date2014-10-09T18:48:31Z
dc.date2014-10-09T18:48:31Z
dc.date2013
dc.date.accessioned2023-09-26T22:15:55Z
dc.date.available2023-09-26T22:15:55Z
dc.identifierVASCONCELOS, J. F. et al. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. FASEB Journal, v. 27, n. 12, p. 4691-4702, 2013.
dc.identifier1530-6860
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8571
dc.identifier10.1096/fj.13-229351
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8877036
dc.descriptionChagas disease, caused by Trypanosoma cruzi infection, is a leading cause of heart failure in Latin American countries. In a previous study, we showed beneficial effects of granulocyte colony-stimulating factor (G-CSF) administration in the heart function of mice with chronic T. cruzi infection. Presently, we investigated the mechanisms by which this cytokine exerts its beneficial effects. Mice chronically infected with T. cruzi were treated with human recombinant G-CSF (3 courses of 200 µg/kg/d for 5 d). Inflammation and fibrosis were reduced in the hearts of G-CSF-treated mice, compared with the hearts of vehicle-treated mice, which correlated with decreased syndecan-4, intercellular adhesion molecule-1, and galectin-3 expressions. Marked reductions in interferon-γ and tumor necrosis factor-α and increased interleukin-10 and transforming growth factor-ß were found after G-CSF administration. Because the therapy did not induce a Th1 to Th2 immune response deviation, we investigated the role of regulatory T (Treg) cells. A significant increase in CD3(+)Foxp3(+) cells was observed in the hearts of G-CSF-treated mice. In addition, a reduction of parasitism was observed after G-CSF treatment. Our results indicate a role of Treg cells in the immunosuppression induced by G-CSF treatment and reinforces its potential therapeutic use for patients with Chagas disease
dc.formatapplication/pdf
dc.languageeng
dc.publisherFederation of American Society of Experimental Biology
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectCytokine therapy
dc.subjectInflammation
dc.subjectFibrosis
dc.subjectTh1 modulation
dc.subjectCardiomiopatia Chagásica/quimioterapia
dc.subjectFator Estimulador de Colônias de Granulócitos/uso terapêutico
dc.subjectImunomodulação
dc.subjectMiocardite/quimioterapia
dc.subjectLinfócitos T Reguladores/efeitos de drogas
dc.subjectAnimais
dc.subjectAntígenos CD3/genética
dc.subjectCardiomiopatia Chagásica/imunologia
dc.subjectCitocinas/genética
dc.subjectFibrose/quimioterapia
dc.subjectFatores de Transcrição Forkhead/genética
dc.subjectGalectina 3/genética
dc.subjectFator Estimulador de Colônias de Granulócitos/administração & dosagem
dc.subjectHumanos
dc.subjectInjeções Intraperitoneais
dc.subjectMolécula 1 de Adesão Intercelular/genética
dc.subjectCamundongos Endogâmicos C57BL
dc.subjectMiocárdio/imunologia
dc.subjectSindecana-4/genética
dc.subjectTranscrição Genética
dc.subjectTrypanosoma cruzi/efeitos de drogas
dc.titleAdministration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
dc.typeArticle


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