Fast high yield of pure Leishmania (Leishmania) infantum axenic amastigotes and their infectivity to mouse macrophages

dc.creatorCosta, Juliana Dias
dc.creatorSoares, Renata
dc.creatorFinkelstein, Léa Cysne
dc.creatorCôrte-Real, Suzana
dc.creatorMeirelles, Maria de Nazareth
dc.creatorPorrozzi, Renato
dc.date2018-08-23T14:55:31Z
dc.date2018-08-23T14:55:31Z
dc.date2009
dc.date.accessioned2023-09-26T22:15:14Z
dc.date.available2023-09-26T22:15:14Z
dc.identifierCOSTA, Juliana Dias; et al. Fast high yield of pure Leishmania (Leishmania) infantum axenic amastigotes and their infectivity to mouse macrophages. Parasitol. Res., v.105, p.227-236, Mar. 2009.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28330
dc.identifier10.1007/s00436-009-1390-4
dc.identifier1432-1955
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876880
dc.descriptionLeishmania (L.) infantum (syn. Leishmania chagasi) is a dimorphic protozoan parasite that lives in promastigote and amastigote form in its sandfly vector and mammalian hosts, respectively. Here, we describe an in vitro culture system for the generation of a pure population of L. infantum axenic amastigotes after only 4 days incubation in culture medium supplemented with fetal calf serum, human urine, L: -glutamine, and HEPES at 37 masculineC (pH 5.5). Ultrastrutural analysis and infection assays in two macrophage populations (Kupffer cells (KUP) and peritoneal macrophages (PM)) infected with axenic amastigotes demonstrated that they maintained morphological and biochemical (A2 expression) features and a similar infection pattern to tissue-derived L. infantum amastigotes. The susceptibility of the macrophage lines to axenic or tissue-derived amastigotes and promastigotes was investigated. We found a completely different susceptibility profile for KUP and PM. Liver macrophages, both KUP and immigrant macrophages, are intimately involved in the response to L. infantum infection; this difference in susceptibility is probably related to their capacity to eliminate these parasites. Our in vitro system was thus able to generate axenic amastigotes that resemble tissue-derived amastigotes both in morphology and infectivity pattern; this will help in further investigation of the biological characteristics of the host-parasite relationship as well as the process of pathogenesis.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectLeishmania infantum
dc.subjectamastigotas axênicas
dc.subjectinfectividade
dc.subjectMacrófagos
dc.subjectCamundongos
dc.subjectLeishmania (Leishmania) infantum
dc.subjectaxenic amastigotes
dc.subjectinfectivity
dc.subjectmacrophages
dc.subjectmouse
dc.titleFast high yield of pure Leishmania (Leishmania) infantum axenic amastigotes and their infectivity to mouse macrophages
dc.typeArticle


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