Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells

dc.creatorPicchi‑Constante, Gisele Fernanda Assine
dc.creatorGuerra‑Slompo, Eloise Pavão
dc.creatorTahira, Ana Carolina
dc.creatorAlcantara, Monica Visnieski
dc.creatorAmaral, Murilo Sena
dc.creatorFerreira, Arthur Schveitzer
dc.creatorBatista, Michel
dc.creatorBatista, Cassiano Martin
dc.creatorGoldenberg, Samuel
dc.creatorVerjovski‑Almeida, Sergio
dc.creatorZanchin, Nilson Ivo Tonin
dc.date2021-11-24T19:02:33Z
dc.date2021-11-24T19:02:33Z
dc.date2021
dc.date.accessioned2023-09-26T22:14:55Z
dc.date.available2023-09-26T22:14:55Z
dc.identifierPICCHI-CONSTANTE, Gisele Fernanda Assine et al. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells. Scientific Reports, v.11, n. 21671, p. 1–18, 2021.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50054
dc.identifier10.1038/s41598-021-01080-1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876808
dc.descriptionTrypanosoma cruzi—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architecture and chromatin structure along their complex life cycle which, combined with a remarkable set of reversible histone post-translational modifcations, indicate that chromatin is also a target for control of gene expression and diferentiation signals in these organisms. Chromatin-modifying enzymes have a direct impact on gene expression programs and DNA metabolism. In this work, we have investigated the function of T. cruzi histone deacetylase 4 (TcHDAC4). We show that, although TcHDAC4 is not essential for viability, metacyclic trypomastigote TcHDAC4 null mutants show a thin cell body and a round and less condensed nucleus located very close to the kinetoplast. Sixty-four acetylation sites were quantitatively evaluated, which revealed H2AT85ac, H4K10ac and H4K78ac as potential target sites of TcHDAC4. Gene expression analyses identifed three chromosomes with overrepresented regions of diferentially expressed genes in the TcHDAC4 knockout mutant compared with the wild type, showing clusters of either up or downregulated genes. The adjacent chromosomal location of some of these genes indicates that TcHDAC4 participates in gene expression regulation during T. cruzi diferentiation.
dc.formatapplication/pdf
dc.languagepor
dc.publisherNature Research
dc.rightsopen access
dc.subjectGene Expression Regulation
dc.subjectHistone Deacetylases
dc.subjectCell Differentiation
dc.subjectRegulación de la Expresión Génica
dc.subjectHistona Desacetilasas
dc.subjectDiferenciación Celular
dc.subjectRégulation de l'expression des gènes
dc.subjectHistone deacetylases
dc.subjectDifférenciation cellulaire
dc.subjectTrypanosoma cruzi
dc.subjectRegulação da Expressão Gênica
dc.subjectHistona Desacetilases
dc.subjectDiferenciação Celular
dc.titleMetacyclogenesis defects and gene expression hallmarks of histone deacetylase 4‑deficient Trypanosoma cruzi cells
dc.typeArticle


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