Duguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo

dc.creatorCosta, Emmanoel V.
dc.creatorSouza, César A. S. de
dc.creatorGalvão, Alexandre F. C.
dc.creatorSilva, Valdenizia R.
dc.creatorSantos, Luciano de S.
dc.creatorDias, Rosane B.
dc.creatorRocha, Clarissa A. Gurgel
dc.creatorSoares, Milena B. P.
dc.creatorSilva, Felipe M. A. da
dc.creatorKoolen, Hector H. F.
dc.creatorBezerra, Daniel P.
dc.date2022-12-21T18:32:18Z
dc.date2022-12-21T18:32:18Z
dc.date2022
dc.date.accessioned2023-09-26T22:13:58Z
dc.date.available2023-09-26T22:13:58Z
dc.identifierCosta, Emmanoel V. et al. Duguetia pycnastera sandwith (Annonaceae) leaf essential oil inhibits HepG2 cell growth in vitro and in vivo. Molecules, v. 27, p. 1-14, 2022.
dc.identifier1420-3049
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/56141
dc.identifier10.3390/molecules 27175664
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876586
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
dc.descriptionAbstract: Duguetia pycnastera Sandwith (Annonaceae) is a tropical tree that can be found in the Guyanas, Bolivia, Venezuela, and Brazil. In Brazil, it is popularly known as “ata”, “envira”, “envirapreta”, and “envira-surucucu”. In the present work, we investigated the in vitro and in vivo HepG2 cell growth inhibition capacity of D. pycnastera leaf essential oil (EO). The chemical composition of the EO was determined by GC–MS and GC–FID analyses. The alamar blue assay was used to examine the in vitro cytotoxicity of EO in cancer cell lines and non-cancerous cells. In EO-treated HepG2 cells, DNA fragmentation was measured by flow cytometry. The in vivo antitumor activity of the EO was assessed in C.B-17 SCID mice xenografted with HepG2 cells treated with the EO at a dosage of 40 mg/kg. Chemical composition analysis displayed the sesquiterpenes -gurjunene (26.83%), bicyclogermacrene (24.90%), germacrene D (15.35%), and spathulenol (12.97%) as the main EO constituents. The EO exhibited cytotoxicity, with IC50 values ranging from 3.28 to 39.39 g/mL in the cancer cell lines SCC4 and CAL27, respectively. The cytotoxic activity of the EO in non-cancerous cells revealed IC50 values of 16.57, 21.28, and >50 g/mL for MRC-5, PBMC, and BJ cells, respectively. An increase of the fragmented DNA content was observed in EO-treated HepG2 cells. In vivo, EO displayed tumor mass inhibition activity by 47.76%. These findings imply that D. pycnastera leaf EO may have anti-liver cancer properties.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectDuguetia pycnastera
dc.subjectÓleo essencial
dc.subjectCélulas HepG2
dc.subjectCitotóxico
dc.subjectantitumoral
dc.subjectDuguetia pycnastera
dc.subjectEssential oil
dc.subjectHepG2 cells
dc.subjectCytotoxic
dc.subjectAntitumor
dc.subjectÓleos Voláteis
dc.subjectCélulas Hep G2
dc.subjectCitotoxinas
dc.titleDuguetia pycnastera sandwith (Annonaceae) leaf essential oil Inhibits HepG2 cell growth In vitro and in vivo
dc.typeArticle


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