Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.

dc.creatorReis, Eliana Almeida Gomes
dc.creatorCarmo, Theomira Mauadie de Azevedo
dc.creatorAthanazio, Rodrigo
dc.creatorReis, Mitermayer Galvão dos
dc.creatorHarn Junior, Donald A
dc.date2014-04-24T11:49:58Z
dc.date2014-04-24T11:49:58Z
dc.date2008
dc.date.accessioned2023-09-26T22:13:40Z
dc.date.available2023-09-26T22:13:40Z
dc.identifierREIS, E. A. G. et al. Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile. Scandinavian Journal of Immunology, v. 68, n. 2, p. 169-176, 2008.
dc.identifier1365-3083
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7530
dc.identifier10.1111/j.1365-3083.2008.02131.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876517
dc.descriptionWe evaluated the ability of naïve monocyte-derived dendritic cells (DC) to sensitize autologous peripheral blood mononuclear cells (PBMC) to the schistosome vaccine candidate MAP4 using a priming in vitro (PIV) assay. MAP4 is a multiple antigen peptide containing B- and T-cell epitopes derived from the glycolytic enzyme triose phosphate isomerase. PBMC primed and restimulated with MAP4 first and secondary recalls (MAP4 PIV cells) were examined for cell phenotype and cytokine production. We found that after the first recall stimulation with MAP4, the major cell population was predominantly CD4(+) T-cell subsets (68.5%), CD8(+high) (16%) and CD19(+) (10%). Additionally, MAP4 PIV cells significantly expressed CD4(+)-HLA-DR(+), -CD54(+), -CD45RO(+) (P < 0.0001) and -CD25(+) (P < 0.0004) together with significant expression of CD80(+) on CD19(+) B cells (P < 0.007). Cytokine production from activated MAP4 PIV cells was predominantly Th1-like, consisting mainly of IFN-gamma. Interestingly, IFN-gamma production was suppressed when Schistosoma mansoni-soluble egg antigen (SEA) was added to a MAP4 PIV cell culture. Furthermore, addition of MAP4 to a SEA PIV cell culture significantly reduced secretion of IL-10. The present findings add to the knowledge gained from studies in the mouse model, and our results show that naïve donor DC, sensitized with MAP4, were able to prime and clonally expand MAP4-specific T cells towards a Th1-type response.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBlackwell Publishing Ltd
dc.rightsopen access
dc.subjectAntígenos de Helmintos/imunologia
dc.subjectCitocinas/imunologia
dc.subjectSchistosoma mansoni/imunologia
dc.subjectSubpopulações de Linfócitos T/imunologia
dc.subjectCélulas Th1/imunologia
dc.subjectTriose-Fosfato Isomerase/imunologia
dc.subjectAnimais
dc.subjectCitocinas/biossíntese
dc.subjectCélulas Dendríticas/imunologia
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectCitometria de Fluxo
dc.subjectHumanos
dc.subjectAtivação Linfocitária/imunologia
dc.subjectPeptídeos/imunologia
dc.titleSchistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
dc.typeArticle


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