Leishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred mice

dc.creatorSouza, Celeste da Silva Freitas de
dc.creatorCalabrese, Kátia da Silva
dc.creatorSilva, Ana Lúcia Abreu
dc.creatorCarvalho, Luiz Otávio Pereira
dc.creatorCardoso, Flávia de Oliveira
dc.creatorDorval, Maria Elizabeth Moraes Cavalheiros
dc.creatorOshiro, Elisa Teruya
dc.creatorQuaresma, Patrícia Flávia
dc.creatorGontijo, Célia Maria Ferreira
dc.creatorPacheco, Raquel da Silva
dc.creatorRossi, Maria Isabel Doria
dc.creatorCosta, Sylvio Celso Gonçalves da
dc.creatorValle, Tânia Zaverucha do
dc.date2019-04-26T15:59:49Z
dc.date2019-04-26T15:59:49Z
dc.date2018
dc.date.accessioned2023-09-26T22:13:12Z
dc.date.available2023-09-26T22:13:12Z
dc.identifierSOUZA, Celeste da Silva Freitas de et al. Leishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred mice. Histology and Histopathology, v. 33, n. 7, p. 705-716, 2018.
dc.identifier0213-3911
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32787
dc.identifier10.14670/HH-11-965
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876422
dc.descriptionLeishmania amazonensis is a major etiological agent of human cutaneous leishmaniasis in the Americas; nevertheless there are some reports of this species causing visceral disease in dogs and men. In the present work we have studied a Leishmania strain isolated from a human case of visceral leishmaniasis. We have infected different mouse strains and analyzed the development of the disease, studying the parasite's ability to visceralize and whether this ability is influenced by host genetics. Female BALB/c, C57BL/6, C57BL/10, CBA, DBA/2, and C3H/He mice were subcutaneously infected with 10(4) L. amazonensis amastigotes. BALB/c, C57BL/6 and C57BL/10 mice were found to be very susceptible to infection, showing lesions that developed to necrosis and ulceration. CBA mice developed a late but severe lesion. DBA/2 mice developed only discrete lesions, while C3H/He mice did not develop any lesions. All mouse strains except C3H/He showed some degree of visceralization, presenting parasites in the spleen, while BALB/c, C57BL/6 and CBA presented parasites also in the liver. Moreover, most of the strains presented high parasite load at the infection site, whereas DBA and C3H/He mice showed low or no parasite load 90 days after infection, respectively. Histopathology corroborates the results, showing that susceptible mice presented an inflammatory reaction with parasites in the skin, lymph nodes and spleen, while strains that are more resistant presented low parasitism and discrete inflammatory reaction. Results indicate that this isolate is extremely virulent, can easily visceralize and that the pathogenesis of leishmaniasis is, at least in part, related to the genetic background of the host.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Murcia
dc.rightsrestricted access
dc.subjectLeishmania amazonensis
dc.subjectViscerização
dc.subjectAnálises histopatológicas
dc.subjectSusceptibilidade
dc.subjectModelo murino
dc.subjectResistência
dc.subjectLeishmania amazonensis
dc.subjectVisceralization
dc.subjectHistopathological analysis
dc.subjectSusceptibility
dc.subjectMurine model
dc.subjectResistance
dc.titleLeishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred mice
dc.typeArticle


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