Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.

dc.creatorAlmeida, Taís Fontoura de
dc.creatorPalma, Luana Carneiro
dc.creatorMendez, Lucas C
dc.creatorDutra, Alberto Augusto Noronha
dc.creatorVeras, Patrícia Sampaio Tavares
dc.date2014-05-30T11:44:46Z
dc.date2014-05-30T11:44:46Z
dc.date2012
dc.date.accessioned2023-09-26T22:12:48Z
dc.date.available2023-09-26T22:12:48Z
dc.identifierALMEIDA, T. F. et al. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunology, v. 34, n. 10, p. 492-498, 2012.
dc.identifier1365-3024
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7728
dc.identifier10.1111/j.1365-3024.2012.01384.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876328
dc.descriptionCBA mouse macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. It has been established that some Leishmania species are destroyed by reactive oxygen species (ROS). However, other species of Leishmania exhibit resistance to ROS or even down-modulate ROS production. We hypothesized that L. amazonensis-infected macrophages reduce ROS production soon after parasite-cell interaction. Employing a highly sensitive analysis technique based on chemiluminescence, the production of superoxide (O(·-)(2)) and hydrogen peroxide (H(2)O(2)) by L. major- or L. amazonensis-infected CBA macrophages were measured. L. major induces macrophages to release levels of (O(·-)(2)) 3·5 times higher than in uninfected cells. This (O(·-)(2)) production is partially dependent on NADPH oxidase (NOX) type 2. The level of accumulated H(2)O(2) is 20 times higher in L. major-than in L. amazonensis-infected cells. Furthermore, macrophages stimulated with L. amazonensis release amounts of ROS similar to uninfected cells. These findings support previous studies showing that CBA macrophages are effective in controlling L. major infection by a mechanism dependent on both (O(·-)(2)) production and H(2)O(2) generation. Furthermore, these data reinforce the notion that L. amazonensis survive inside CBA macrophages by reducing ROS production during the phagocytic process.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBlackwell Publishing Ltd
dc.rightsopen access
dc.subjectLeishmania
dc.subjectMacrophage
dc.subjectReactive oxygen intermediates
dc.subjectLeishmania mexicana/imunologia
dc.subjectMacrófagos/imunologia
dc.subjectMacrófagos/parasitologia
dc.subjectEspécies de Oxigênio Reativas/metabolismo
dc.subjectAnimais
dc.subjectPeróxido de Hidrogênio/análise
dc.subjectLeishmania/imunologia
dc.subjectLeishmania major/imunologia
dc.subjectMedições Luminescentes
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos CBA
dc.subjectNADPH Oxidase/metabolismo
dc.subjectSuperóxidos/análise
dc.titleLeishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
dc.typeArticle


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