Development of nuclear DNA probes for the typing of Trypanososma cruzi

dc.creatorTakeuchi, A. Mayumi
dc.creatorTraub-Cseko, Yara M.
dc.date2020-09-18T18:35:11Z
dc.date2020-09-18T18:35:11Z
dc.date1991
dc.date.accessioned2023-09-26T22:12:25Z
dc.date.available2023-09-26T22:12:25Z
dc.identifierTAKEUCHI, A. Mayumi; TRAUB-CSEKO, Yara M. Development of nuclear DNA probes for the typing of Trypanososma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 3, p. 307-315, July/Sept. 1991.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43457
dc.identifier10.1590/S0074-02761991000300005
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876240
dc.descriptionWe have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectSondagens aleatórias
dc.subjectTaxonomia numérica
dc.subjectDistensões
dc.subjectTrypanosoma cruzi
dc.subjectRandom probes
dc.subjectTyping
dc.subjectStrains
dc.subjectNumerical taxonomy
dc.subjectDistenções
dc.titleDevelopment of nuclear DNA probes for the typing of Trypanososma cruzi
dc.typeArticle


Este ítem pertenece a la siguiente institución