Production and Characterization of IgY against Canine IgG: Prospect of a New Tool for the Immunodiagnostic of Canine Diseases

dc.creatorSantos, Fernanda Nunes
dc.creatorBrm, Beatriz Coutinho
dc.creatorCruz, Paula Borba
dc.creatorMolinaro, Claudia Moraes
dc.creatorSilva, Valmir Laurentino
dc.creatorChaves, Sérgio Augusto de Miranda
dc.date2015-09-21T17:25:17Z
dc.date2015-09-21T17:25:17Z
dc.date2014
dc.date.accessioned2023-09-26T22:11:26Z
dc.date.available2023-09-26T22:11:26Z
dc.identifierSANTOS, Fernanda Nunes; et al. Production and Characterization of IgY against Canine IgG: Prospect of a New Tool for the Immunodiagnostic of Canine Diseases. Braz. arch. biol. technol., v.57, n.4, p. 523-531, 2014.
dc.identifier1516-8913
dc.identifier110.1590/S1516-89132014005000020
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/11689
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8876002
dc.descriptionThis study describes the production of a new avian polyclonal antibody (IgY) against canine IgG, as another tool for the immunodiagnostic using IgY technology. The immunization protocol caused neither deaths nor pathologies, and no decline in egg laying capacity was detected. The total concentration of isolated IgY was constant, without significant difference (p> 0.05), with average of 97.55 mg of IgY/yolk. The IgY revealed a strong sensitivity and specificity in recognition against canine IgG by ELISA. After the immunization, there was a significant increase in the production of IgY specific from the first to the second month (p <0.05), reaching a stable peak without decrease in the production by the end of the analysis period (p > 0.05). The IgY demonstrated a suitable specificity in Western blot against the purified and serum canine IgG, not enabling recognition of canine IgM or IgG of other animal species. The specific IgY in the egg yolks of immunized hens proved to be a molecule with an appropriate purity and desired specificity against the immunizing antigen. Moreover, its constant production in large quantities during the four months analyzed indicated that IgY antibody production technology could be considered as an excellent alternative to the standard methods.
dc.formatapplication/pdf
dc.languageeng
dc.publisherTecpar
dc.rightsopen access
dc.subjectAnticorpos
dc.subjectGalinha
dc.subjectCanino
dc.subjectIgY polyclonal antibodies
dc.subjectChicken antibodies
dc.subjectHen immunization
dc.subjectCanine IgG
dc.titleProduction and Characterization of IgY against Canine IgG: Prospect of a New Tool for the Immunodiagnostic of Canine Diseases
dc.typeArticle


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