Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification

dc.creatorTrilles, Luciana
dc.creatorWang, Bin
dc.creatorFiracative, Carolina
dc.creatorLazéra, Márcia dos Santos
dc.creatorWanke, Bodo
dc.creatorMeyer, Wieland
dc.date2015-04-09T16:51:37Z
dc.date2015-04-09T16:51:37Z
dc.date2014
dc.date.accessioned2023-09-26T22:10:40Z
dc.date.available2023-09-26T22:10:40Z
dc.identifierTRILLES, Luciana et al. Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification. Plos One, v. 9, n. 4, p. 1-8, Apr. 2014.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9940
dc.identifier10.1371/journal.pone.0094648
dc.identifier1932-6203
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875823
dc.descriptionThe agents of cryptococcosis C. neoformans and C. gattii are important agents of meningoencephalitis in immunocompromised and immunocompetent hosts, respectively. They are grouped into eight major molecular types, VNI-VNIV for C. neoformans and VGI-VGIV for C. gattii. These major molecular types differ in their host range, epidemiology, antifungal susceptibility and geographic distribution. To enable a rapid identification of the major molecular types and potential hybrids within the two species specific probes based on the PLB1 gene in combination with hyperbranched rolling circle amplification (HRCA) were developed. HRCA was applied to 76 cryptococcal strains, 10 strains each representing the 7 haploid major molecular types, 4 VNIII hybrid strains and 2 inter-species hybrid strains. All strains were correctly identified to the major molecular type and or hybrid type using HRCA alone. To increase the sensitivity a semi-nested PCR step was developed, which will enable the identification of the molecular types/hybrids directly from clinical samples, harboring a low copy number of DNA (40 copies). Thus, HRCA based on the PLB1 locus alone and in combination with a semi-nested PCR showed to be a specific and sensitive methodology, with a great potential to be used on clinical specimens for the direct diagnosis of the agents of cryptococcosis, including hybrid strains, enabling a rapid and patient tailored treatment choice of this disease.
dc.formatapplication/pdf
dc.languagepor
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectCryptococcus neoformans
dc.subjectCryptococcus gattii
dc.subjectMeningoencephalitis
dc.subjectPolymerase chain reaction
dc.titleIdentification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
dc.typeArticle


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