Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.

dc.creatorSchriefer, Albert
dc.creatorMaltez, J. R
dc.creatorSilva, N
dc.creatorStoeckle, M.Y
dc.creatorBarral Netto, Manoel
dc.creatorRiley, Lee Woodland
dc.date2012-10-05T18:51:53Z
dc.date2012-10-05T18:51:53Z
dc.date1999
dc.date.accessioned2023-09-26T22:10:20Z
dc.date.available2023-09-26T22:10:20Z
dc.identifierSCHRIEFER, A. et al. Expression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain. Vaccine, v. 17, n. 7-8, p. 770-8, feb. 1999.
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5652
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875740
dc.descriptionEnteropathogenic Escherichia coli (EPEC) is a major cause of childhood diarrhea in developing countries and is a leading cause of severe diarrheal illness among Brazilian infants. As one approach to constructing a vaccine candidate against diarrhea caused by EPEC, we evaluated whether the pilin subunit (BfpA) of the bundle-forming pilus (BFP) could be expressed by a live Salmonella vaccine strain. Several copies of the coding region of BfpA (bfpA) were amplified by PCR from a preparation of the EAF plasmid of EPEC strain B171 and cloned into plasmid vectors. An intact copy of bfpA was subcloned into the heat inducible prokaryotic expression vector pCYTEXP1, and the resulting pBfpA was used to transform the aroA S. typhimurium strain SL3261, generating SL3261(pBfpA). The recombinant vaccine strain was able to express, but not to process, rBfpA as evidenced by a prominent 21 kDa protein that crossreacted with anti-BFP antiserum found only in extracts of heat-treated SL3261(pBfpA), but not in strains of untreated SL3261(pBfpA) or SL3261 not carrying the plasmid. Furthermore, rBfpA accumulation was not toxic to the Salmonella host, as evidenced by similar plating efficiencies between induced and uninduced strains of SL3261(pBfpA). Finally, SL3261(pBfpA) orally administered to BALB/c mice was capable of eliciting a sustained and vigorous humoral immune response to BfpA, achievable even with a single oral dose of approximately 10(9) organisms. Therefore, this pilin product may serve as a potential immunogen as part of a live combined vaccine strategy to prevent two of the major public health problems in Brazil--salmonellosis and EPEC childhood diahrrea.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectEPEC
dc.subjectBFP
dc.subjectVaccine
dc.subjectOral
dc.subjectProteínas da Membrana Bacteriana Externa/genética
dc.subjectVacinas Bacterianas/imunologia
dc.subjectProteínas de Escherichia coli
dc.subjectEscherichia coli/genética
dc.subjectEscherichia coli/imunologia
dc.subjectFímbrias Bacterianas/imunologia
dc.subjectProteínas de Membrana/genética
dc.subjectVacinas contra Salmonella
dc.subjectSalmonella typhimurium/imunologia
dc.subjectVacinas Tíficas-Paratíficas
dc.subjectAdministração Oral
dc.subjectAnimais
dc.subjectAnticorpos Antibacterianos/biossíntese
dc.subjectEspecificidade de Anticorpos
dc.subjectProteínas da Membrana Bacteriana
dc.subjectExterna/biossíntese
dc.subjectVacinas Bacterianas/genética
dc.subjectClonagem Molecular
dc.subjectEscherichia coli/patogenicidade
dc.subjectFeminino
dc.subjectProteínas de Fímbrias
dc.subjectFímbrias Bacterianas/genética
dc.subjectRegulação Bacteriana da Expressão
dc.subjectGênica/imunologia
dc.subjectVetores Genéticos/imunologia
dc.subjectProteínas de Membrana/imunologia
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectSalmonella typhimurium/genética
dc.subjectTransformação Bacteriana
dc.subjectVacinas Atenuadas/genética
dc.subjectVacinas Atenuadas/imunologia
dc.subjectVacinas Sintéticas/imunologia
dc.titleExpression of a pilin subunit BfpA of the bundle-forming pilus of enteropathogenic Escherichia coli in an aroA live salmonella vaccine strain.
dc.typeArticle


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