In vitro selection of Phytomonas serpens cells resistant to the calpain inhibitor MDL28170: alterations in fitness and expression of the major peptidases and efflux pumps

dc.creatorOliveira, Simone S. C.
dc.creatorGonçalves, Inês C.
dc.creatorEnnes-Vidal, Vitor
dc.creatorLopes, Angela H. C. S.
dc.creatorMenna-Barreto, Rubem F. S.
dc.creatord`Avila-Levy, Claudia M.
dc.creatorSantos, André L. S.
dc.creatorBranquinha, Marta H.
dc.date2018-09-13T18:09:59Z
dc.date2018-09-13T18:09:59Z
dc.date2018
dc.date.accessioned2023-09-26T22:10:17Z
dc.date.available2023-09-26T22:10:17Z
dc.identifierGESTO, João Silveira Moledo; et al. In tune with nature: Wolbachia does not prevent pre-copula acoustic communication in Aedes aegypti. Parasites & Vectors, v.11, n.109, 8p, 2018.
dc.identifier1469-8161
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28762
dc.identifier10.1017/S0031182017001561
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875728
dc.descriptionThe species Phytomonas serpens is known to express some molecules displaying similarity to those described in trypanosomatids pathogenic to humans, such as peptidases from Trypanosoma cruzi (cruzipain) and Leishmania spp. (gp63). In this work, a population of P. serpens resistant to the calpain inhibitor MDL28170 at 70 µ m (MDLR population) was selected by culturing promastigotes in increasing concentrations of the drug. The only relevant ultrastructural difference between wild-type (WT) and MDLR promastigotes was the presence of microvesicles within the flagellar pocket of the latter. MDLR population also showed an increased reactivity to anti-cruzipain antibody as well as a higher papain-like proteolytic activity, while the expression of calpain-like molecules cross-reactive to anti-Dm-calpain (from Drosophila melanogaster) antibody and calcium-dependent cysteine peptidase activity were decreased. Gp63-like molecules also presented a diminished expression in MDLR population, which is probably correlated to the reduction in the parasite adhesion to the salivary glands of the insect vector Oncopeltus fasciatus. A lower accumulation of Rhodamine 123 was detected in MDLR cells when compared with the WT population, a phenotype that was reversed when MDLR cells were treated with cyclosporin A and verapamil. Collectively, our results may help in the understanding of the roles of calpain inhibitors in trypanosomatids.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherCambridge University Press
dc.rightsrestricted access
dc.subjectResistência
dc.subjectPeptidases
dc.subjectPhytomonas
dc.subjectproteínas semelhantes a calpaína
dc.subjectPhytomonas
dc.subjectcalpain-like proteins
dc.subjectresistance
dc.subjectpeptidases
dc.titleIn vitro selection of Phytomonas serpens cells resistant to the calpain inhibitor MDL28170: alterations in fitness and expression of the major peptidases and efflux pumps
dc.typeArticle


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