Comparison of DNA vaccines producing HIV-1 Gag and LAMP/Gag chimera in rhesus macaques reveals antigen-specific T-cell responses with distinct phenotypes

dc.creatorValentin, Antonio
dc.creatorChikhlikar, Priya
dc.creatorPatel, Vainav
dc.creatorRosati, Margherita
dc.creatorMaciel, Milton
dc.creatorChang, Kern-Hee
dc.creatorSilvera, Peter
dc.creatorFelber, Barbara K.
dc.creatorPavlakis, George N.
dc.creatorAugust, J. Thomas
dc.creatorMarques, Ernesto T. A.
dc.date2018-08-06T12:21:44Z
dc.date2018-08-06T12:21:44Z
dc.date2009
dc.date.accessioned2023-09-26T22:10:13Z
dc.date.available2023-09-26T22:10:13Z
dc.identifierVALENTIN, A. et al. Comparison of DNA vaccines producing HIV-1 Gag and LAMP/Gag chimera in rhesus macaques reveals antigen-specific T-cell responses with distinct phenotypes. Vaccine, v. 27, n. 35, p. 4840–4849, 30 jul. 2009.
dc.identifier1873-2518
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27852
dc.identifier10.1016/j.vaccine.2009.05.093
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875714
dc.descriptionEsta pesquisa foi apoiada pelo Programa de Pesquisa Intramural do NIH, National Cancer Institute, Center for Cancer Research.
dc.descriptionOptimized DNA expression vectors encoding the native HIV-1 Gag or a fusion of Gag with the lysosomal membrane associated protein 1 (LAMP) were compared for immunogenicity upon intramuscular DNA delivery in rhesus macaques. Both vaccines elicited CD4(+) T-cell responses, but with significant differences in the phenotype of the Gag-specific cells: the native Gag induced CD4(+) responses with a phenotype of central memory-like T cells (CD28(+) CD45RA(-)), whereas the LAMP/Gag chimera induced CD4(+) responses with effector memory phenotype (CD28(-) CD45RA(-)). Antigen-specific T cells producing both IFN-gamma and TNFalpha were found in the animals receiving the native Gag, whereas the LAMP/Gag chimera induced humoral responses faster. These results demonstrate that modification of intracellular Gag trafficking results in the induction of distinct immune responses. Combinations of DNA vectors encoding both forms of antigen may be more potent in eliciting anti-HIV-1 immunity.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectMemória central
dc.subjectMemória efectora
dc.subjectCD4
dc.subjectCD8
dc.subjectCélulas T
dc.subjectIFN-gama
dc.subjectCentral memory
dc.subjectEffector memory
dc.subjectCD4
dc.subjectCD8
dc.subjectT cells
dc.subjectIFN-gamma
dc.subjectTNF-alpha
dc.subjectIL-2
dc.subjectVacinas contra a AIDS / genética
dc.subjectVacinas contra a AIDS / imunologia
dc.subjectAdjuvantes Imunológicos / genética
dc.subjectAdjuvantes Imunológicos / Farmacologia
dc.subjectAnimais
dc.subjectCD28 Antígenos / análise
dc.subjectLinfócitos T CD4-Positivos / imunologia
dc.subjectAnticorpos HIV / sangue
dc.subjectHIV-1 / genética
dc.subjectHIV-1 / imunologia
dc.subjectInjeções Intramusculares
dc.subjectInterferon-gama / secreção
dc.subjectAntígenos comuns de leucócitos / análise
dc.subjectGlicoproteínas de Membrana Associadas a Lisossomos / genética
dc.subjectGlicoproteínas Associadas à Membrana Lisossômica / farmacologia
dc.subjectMacaca mulatta
dc.subjectMasculino
dc.subjectSubgrupos de linfócitos T / imunologia
dc.subjectFator de Necrose Tumoral alfa / secreção
dc.subjectVacinas , DNA / genética
dc.subjectVacinas Sintéticas / imunologia
dc.subjectgag Produtos Gene, Vírus da Imunodeficiência Humana / genética
dc.subjectgag Produtos Gene, Vírus da Imunodeficiência Humana / imunologia
dc.titleComparison of DNA vaccines producing HIV-1 Gag and LAMP/Gag chimera in rhesus macaques reveals antigen-specific T-cell responses with distinct phenotypes
dc.typeArticle


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