| dc.creator | Santos, Gisele Graça Leite dos | |
| dc.creator | Silva, Luciana Lyra Casais e | |
| dc.creator | Soares, Milena Botelho Pereira | |
| dc.creator | Villarreal, Cristiane Flora | |
| dc.date | 2013-11-14T18:05:41Z | |
| dc.date | 2013-11-14T18:05:41Z | |
| dc.date | 2012 | |
| dc.date.accessioned | 2023-09-26T22:10:04Z | |
| dc.date.available | 2023-09-26T22:10:04Z | |
| dc.identifier | SANTOS, G. G. L. dos et al. Antinociceptive properties of Micrurus lemniscatus venom. Toxicon, v. 60, p. 1005–1012, 2012. | |
| dc.identifier | 0041-0101 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/7235 | |
| dc.identifier | doi.org/10.1016/j.toxicon.2012.07.003 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8875674 | |
| dc.description | The therapeutic potential of snake venoms for pain control has been previously demonstrated.
In the present study, the antinociceptive effects of Micrurus lemniscatus venom
(MlV) were investigated in experimental models of pain. The antinociceptive activity of
MIV was evaluated using the writhing, formalin, and tail flick tests. Mice motor performance
was assessed in the rota rod and open field tests. In a screening test for new
antinociceptive substances – the writhing test – oral administration of MlV (19.7–1600 mg/
kg) produced significant antinociceptive effect. The venom (1600 mg/kg) also inhibited
both phases of the formalin test, confirming the antinociceptive activity. The administration
of MlV (1600 mg/kg) did not cause motor impairment in the rota rod and open field
tests, which excluded possible non-specific muscle relaxant or sedative effects of the
venom. The MIV (177–1600 mg/kg) also increases the tail flick latency response, indicating
a central antinociceptive effect for the venom. In this test, the MlV-induced antinociceptive
effect was long-lasting and higher than that of morphine, an analgesic considered the gold
standard. In another set of experiments, the mechanisms involved in the venom-induced
antinociception were investigated through the use of pharmacological antagonists. The
MlV (1600 mg/kg) antinociceptive effect was prevented by naloxone (5 mg/kg), a nonselective
opioid receptor antagonist, suggesting that this effect is mediated by activation
of opioid receptors. In addition, the pre-treatment with the m-opioid receptor antagonist
CTOP (1 mg/kg) blocked the venom antinociceptive effect, while the k-opioid receptor
antagonist nor-BNI (0.5 mg/kg) or the d-opioid receptor antagonist naltrindole (3 mg/kg)
only partially reduced the venom-induced antinociception. The present study demonstrates,
for the first time, that oral administration of M. lemniscatus venom, at doses that
did not induce any motor performance alteration, produced potent and long-lasting
antinociceptive effect mediated by activation of opioid receptors. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Elsevier | |
| dc.rights | open access | |
| dc.subject | Micrurus lemniscatus | |
| dc.subject | Pain | |
| dc.subject | Analgesic | |
| dc.subject | Antinociception | |
| dc.subject | Venom | |
| dc.subject | Opioid system | |
| dc.title | Antinociceptive properties of Micrurus lemniscatus venom | |
| dc.type | Article | |
