An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections

dc.creatorSaldarriaga, Omar A.
dc.creatorCastellanos-Gonzalez, Alejandro
dc.creatorPorrozzi, Renato
dc.creatorBaldeviano, Gerald C.
dc.creatorLescano, Andrés G.
dc.creatorLos Santos, Maxy B. de
dc.creatorFernandez, Olga L.
dc.creatorSaravia, Nancy G.
dc.creatorCosta, Erika
dc.creatorMelby, Peter C.
dc.creatorTravi, Bruno L.
dc.date2016-12-01T14:12:55Z
dc.date2016-12-01T14:12:55Z
dc.date2016
dc.date.accessioned2023-09-26T22:09:46Z
dc.date.available2023-09-26T22:09:46Z
dc.identifierSAIDARRIAGA, Omar A. et al. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLoS Negl Trop Dis , v.10, n.4, e0004638, 12p, Apr. 2016.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/16401
dc.identifier10.1371/journal. pntd.0004638
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875604
dc.descriptionCutaneous and mucosal leishmaniasis is widely distributed in Central and South America. Leishmania of the Viannia subgenus are the most frequent species infecting humans. L. (V.) braziliensis, L. (V.) panamensis are also responsible for metastatic mucosal leishmaniasis. Conventional or real time PCR is a more sensitive diagnostic test than microscopy, but the cost and requirement for infrastructure and trained personnel makes it impractical in most endemic regions. Primary health systems need a sensitive and specific point of care (POC) diagnostic tool. We developed a novel POC molecular diagnostic test for cutaneous leishmaniasis caused by Leishmania (Viannia) spp. Parasite DNA was amplified using isothermal Recombinase Polymerase Amplification (RPA) with primers and probes that targeted the kinetoplast DNA. The amplification product was detected by naked eye with a lateral flow (LF) immunochromatographic strip. The RPA-LF had an analytical sensitivity equivalent to 0.1 parasites per reaction. The test amplified the principal L. Viannia species from multiple countries: L. (V.) braziliensis (n = 33), L. (V.) guyanensis (n = 17), L. (V.) panamensis (n = 9). The less common L. (V.) lainsoni, L. (V.) shawi, and L. (V.) naiffi were also amplified. No amplification was observed in parasites of the L. (Leishmania) subgenus. In a small number of clinical samples (n = 13) we found 100% agreement between PCR and RPA-LF. The high analytical sensitivity and clinical validation indicate the test could improve the efficiency of diagnosis, especially in chronic lesions with submicroscopic parasite burdens. Field implementation of the RPA-LF test could contribute to management and control of cutaneous and mucosal leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectTeste Molecular
dc.subjectLeishmania cutânea
dc.subjectDiagnóstico
dc.subjectCutaneous Leishmania Viannia spp.
dc.subjectMolecular Test
dc.subjectDiagnosis
dc.titleAn Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
dc.typeArticle


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