Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage

dc.creatorTekiel, Valeria
dc.creatorOliveira, Guilherme Corrêa de
dc.creatorOliveira, Rodrigo Corrêa de
dc.creatorSanchez, Daniel
dc.creatorCappa, Stella Maris Gonzalez
dc.date2013-08-21T16:58:25Z
dc.date2013-08-21T16:58:25Z
dc.date2005
dc.date.accessioned2023-09-26T22:09:31Z
dc.date.available2023-09-26T22:09:31Z
dc.identifierTEKIEL, Valeria et al. Chagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage. Acta Trop., v. 94, n. 1, p. 15-24, 2005.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/6812
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875546
dc.descriptionUsing the same mouse strain and two Trypanosoma cruzisub-populations (CA-I and RA) it is possible to induce pathology in different target tissues: skeletal muscle (CA-I) or sciatic nerve and spinal cord (RA). On the other hand, T cells are directly involved in tissue injury in a strain-dependent way, resembling the abnormalities of chronic Chagas’ disease. In the present work, we examined the TCRBV repertoire and the CDR3 sequence polymorphism of T cells infiltrating spinal cord, sciatic nerve and skeletal muscle in chronically infected mice. The TCRBV9 segment was systematically over-represented in the target tissues for eachT. cruzistrain: sciatic nerve and spinal cord in RA and skeletal muscle in CA-I-infected mice. The analysis of CDR3 sequence polymorphism in the same tissues showed a high proportion of identical TCRBV9 clones in RA-infected mice: 66.6% of the TCRBV9 clones found in sciatic nerve and spinal cord expressed one out of four major CDR3 rearrangements. Sequence identity was shared among clones from sciatic nerve and spinal cord, tissues that are also damaged by passive transfer of CD8 + TL. Those observations are consistent with an antigen driven T-cell expansion sequestered at the inflammation site and demonstrate – for the first time – the presence of an oligoclonal repertoire in the antigen recognition site of over-represented T cells in nervous system tissues in chronic Chagas’ disease.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectT-cell receptor
dc.subjectCDR3 polymorphism
dc.subjectClonal expansion
dc.subjectNervous system
dc.titleChagas' disease: TCRBV9 over-representation and sequence oligoclonality in the fine specificity of T lymphocytes in target tissues of damage
dc.typeArticle


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