Role of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate

dc.creatorSilva Filho, Fernando Costa e
dc.creatorElias, Cezar Antonio
dc.creatorSouza, Wanderley de
dc.date2020-08-22T17:45:12Z
dc.date2020-08-22T17:45:12Z
dc.date1987
dc.date.accessioned2023-09-26T22:08:11Z
dc.date.available2023-09-26T22:08:11Z
dc.identifierSILVA FILHO, Fernando Costa e; ELIAS, Cezar Antonio; SOUZA, Wanderley de. Role of divalent cations, pH, cytoskeleton componentes and surface charge on the adhesion of Trichomonas vaginalis to a polystyrene substrate. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 3, p. 379-384, jul./set. 1987.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/42884
dc.identifier10.1590/S0074-02761987000300009
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8875240
dc.descriptionO processo de adesão de três cepas de Trichomonas vaginalis a um substrato de poliestireno foi estudado. Verificou-se que este processo depende do tempo de incubação e do pH da solução salina em que os parasitos se encontram. A maior taxa de adesão foi observada após 60 minutos de incubação a pH 6,6. A adesão é mior se Ca++ ou Mg++ for adicionado ao meio. Tratamento das células em citocalasina B, tripsina ou neuraminidase reduz a adesão enquanto tratamento com poli-L-lisina facilita esta adesão. Incubação dos parasitos ou esferas de poliestireno na presença de poli-L-lisina provoca alterações importantes na carga de superfície.
dc.descriptionhe process of adhesion of three different strains of Trichomonas vaginalis to a polystyrene substrate was analysed. The process of adhesion was dependent on the time of incubation and the pH of the phosphate-buffered solution (PBS) in which the parasites were suspended. The highest indices of adhesion were observed after an incubation time of 60 min at pH 6.6. The adhesion index increased when the parasites were incubated in the presence of culture media or when Ca++ or Mg++ was added to the PBS solution, whereas cytochalasin B, trypsin or neuraminidase reduced adhesion. Incubation of the parasites in the presence of poly-L-lysine facilitated the process of adhesion. Incubation of the parasites or polystyrene beads in the presence of poly-L-lysine led to important changes in their surface charge.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrichomonas vaginalis
dc.subjectAdesão celular
dc.subjectComponentes do citoesqueleto
dc.subjectCarga de superfície
dc.subjectTrichomonas vaginalis
dc.subjectCell adhesion
dc.subjectcytoskeletal components
dc.subjectSurface charge
dc.titleRole of divalent cations, pH, Cytoskeleton components and surface charge on the adhesion of Trycomas Vaginalis to a polystyrene substrate
dc.typeArticle


Este ítem pertenece a la siguiente institución