Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro

dc.creatorSantos, Tamiris A. C.
dc.creatorSilva, Kleiton P.
dc.creatorSouza, Gabriella B.
dc.creatorAlves, Péricles B.
dc.creatorMenna-Barreto, Rubem F. S.
dc.creatorScher, Ricardo
dc.creatorFernandes, Roberta P. M.
dc.date2023-04-24T12:12:50Z
dc.date2023-04-24T12:12:50Z
dc.date2023
dc.date.accessioned2023-09-26T22:07:04Z
dc.date.available2023-09-26T22:07:04Z
dc.identifierSANTOS, Tamiris A. C. et al. Chalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro. Pathogens, v. 12, 423, p. 1 - 13, Mar. 2023.
dc.identifier2076-0817
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/57918
dc.identifier10.3390/ pathogens12030423
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874975
dc.descriptionPhytomonas serpens is a trypanosomatid phytoparasite, found in a great variety of species, including tomato plants. It is a significant problem for agriculture, causing high economic loss. In order to reduce the vegetal infections, different strategies have been used. The biological activity of molecules obtained from natural sources has been widely investigated to treat trypanosomatids infections. Among these compounds, chalcones have been shown to have anti-parasitic and antiinflammatory effects, being described as having a remarkable activity on trypanosomatids, especially in Leishmania species. Here, we evaluated the antiprotozoal activity of the chalcone derivative (NaF) on P. serpens promastigotes, while also assessing its mechanism of action. The results showed that treatment with the derivative NaF for 24 h promotes an important reduction in the parasite proliferation (IC50/24 h = 23.6 4.6 M). At IC50/24 h concentration, the compound induced an increase in reactive oxygen species (ROS) production and a shortening of the unique flagellum of the parasites. Electron microscopy evaluation reinforced the flagellar phenotype in treated promastigotes, and a dilated flagellar pocket was frequently observed. The treatment also promoted a prominent autophagic phenotype. An increased number of autophagosomes were detected, presenting different levels of cargo degradation, endoplasmic reticulum profiles surrounding different cellular structures, and the presence of concentric membranar structures inside the mitochondrion. Chalcone derivatives may present an opportunity to develop a treatment for the P. serpens infection, as they are easy to synthesize and are low in cost. In order to develop a new product, further studies are still necessary.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectTripanossomatídeo
dc.subjectFitoparasita
dc.subjectAtividade antiprotozoária
dc.subjectAutofagia
dc.subjectEspécies que reagem ao oxigênio
dc.subjectTrypanossomatid
dc.subjectPhytoparasite
dc.subjectAntiprotozoal activity
dc.subjectAutophagy
dc.subjectReactive oxygen species
dc.titleChalcone Derivative Induces Flagellar Disruption and Autophagic Phenotype in Phytomonas serpens In Vitro
dc.typeArticle


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