Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil

dc.creatorSouza, Thiara Manuele Alves de
dc.creatorLima, Raquel Curtinhas de
dc.creatorSolórzano, Victor Edgar Fiestas
dc.creatorDamasco, Paulo Vieira
dc.creatorSouza, Luiz José de
dc.creatorSanchez-Arcila, Juan Camilo
dc.creatorGuimarães, Gabriel Macedo Costa
dc.creatorPaiva, Iury Amâncio
dc.creatorLima, Monique da Rocha Queiroz
dc.creatorBruycker-Nogueira, Fernanda de
dc.creatorTomé, Larissa Cristina Teixeira
dc.creatorCoelho, Mariana Rosa Inácio
dc.creatorSilva, Sandro Patroca da
dc.creatorPinto, Luzia Maria de Oliveira
dc.creatorAzeredo, Elzinandes Leal de
dc.creatorSantos, Flavia Barreto dos
dc.date2022-08-12T20:11:41Z
dc.date2022-08-12T20:11:41Z
dc.date2022
dc.date.accessioned2023-09-26T22:06:48Z
dc.date.available2023-09-26T22:06:48Z
dc.identifierSOUZA, Thiara Manuele Alves de et al. Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil. Pathogens, v. 11, n. 245, p. 1-16, Feb. 2022.
dc.identifier2076-0817
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/54629
dc.identifier10.3390/pathogens11020245
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874911
dc.descriptionThe co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and laboratorial aspects of chikungunya suspected cases assisted in RJ during the 2018 outbreak, focusing on the differential diagnosis with dengue and zika. All suspected cases were submitted to molecular and/or serological differential diagnostic approaches to arboviruses. A total of 242 cases suspected of arbovirus infection were investigated and 73.6% (178/242) were molecular and/or serologically confirmed as chikungunya. In RT-qPCR confirmed cases, cycle threshold (Ct) values ranged from 15.46 to 35.13, with acute cases presenting lower values. Chikungunya cases were mainly in females (64%) and the most frequently affected age group was adults between 46 to 59 years old (27%). Polyarthralgia affected 89% of patients, especially in hands and feet. No dengue virus (DENV) and Zika virus (ZIKV) infections were confirmed by molecular diagnosis, but 9.5% (23/242) had serological evidence of DENV exposure by the detection of specific anti-DENV IgM or NS1, and 42.7% (76/178) of chikungunya positive cases also presented recent DENV exposure reflected by a positive anti-DENV IgM or NS1 result. A significantly higher frequency of arthritis (p = 0.023) and limb edema (p < 0.001) was found on patients with CHIKV monoinfection compared to dengue patients and patients exposed to both viruses. Lastly, phylogenetic analysis showed that the chikungunya cases were caused by the ECSA genotype. Despite the triple arboviruses’ epidemic in the state of RJ, most patients with fever and arthralgia investigated here were diagnosed as chikungunya cases, and the incidence of CHIKV/DENV co-detection was higher than that reported in other studies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectArbovírus
dc.subjectChikungunya
dc.subjectEpidemia tripla
dc.subjectDiagnóstico laboratorial diferencial
dc.subjectFilogenia
dc.subjectGenótipo ECSA
dc.subjectArboviruses
dc.subjectChikungunya
dc.subjectTriple epidemic
dc.subjectDifferential laboratorial diagnosis
dc.subjectPhylogeny
dc.subjectECSA genotype
dc.titleWas It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil
dc.typeArticle


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