Mucosal and systemic anti-GAG immunity induced by neonatal immunization with HIV LAMP/gag DNA vaccine in mice

dc.creatorGoldoni, Adriana Letícia
dc.creatorMaciel, Milton
dc.creatorRigato, Paula Ordonhez
dc.creatorPiubelli, Orlando
dc.creatorBrito, Cyro Alves de
dc.creatorMelo, Andrea
dc.creatorMarques, Ernesto Torres
dc.creatorAugust, Joseph Thomas
dc.creatorDuarte, Alberto José da Silva
dc.creatorSato, Maria Notomi
dc.date2019-01-28T15:06:09Z
dc.date2019-01-28T15:06:09Z
dc.date2011
dc.date.accessioned2023-09-26T22:06:48Z
dc.date.available2023-09-26T22:06:48Z
dc.identifierGOLDONI, Adriana Letícia et al. Mucosal and systemic anti-GAG immunity induced by neonatal immunization with HIV LAMP/gag DNA vaccine in mice. Immunobiology, v. 216, n. 4, p. 505–512, 1 abr. 2011.
dc.identifier1878-3279
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31327
dc.identifier10.1016/j.imbio.2010.08.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874910
dc.descriptionVaccines capable of inducing mucosal immunity in early postnatal life until adulthood, protecting early sexual initiation, should be considered as strategies to vaccination against HIV. The HIV-1 GAG protein as a chimera with the lysosome-associated membrane protein (LAMP/gag), encoded by a DNA vaccine, is targeted to the endosomal/lysosomal compartment that contains class II MHC molecules and has been shown to be immunogenic in adult mice. Assuming that one such strategy could help to overcome the immunological immaturity in the early postnatal period, we have evaluated the systemic and mucosal immunogenicity of LAMP/gag immunization in neonatal mice. Intranasal immunization with LAMP/gag vaccine induced higher levels of sIgA and IgG anti-GAG antibodies in intestinal washes than did the gag vaccine. The combination of ID injections and the IN protocol with the chimeric vaccine promoted the increase of Ab levels in sera. Both vaccines induced splenic IFN-γ- secreting cells against GAG peptide pools, as well as in vivo cytotoxic T lymphocyte (CTL) function, and increased the percentage of CD8+ T cells to the immunodominant class I peptide in gut and spleen. However, only the chimeric vaccine was able to enhance Th1/Th2 cytokine secretion in response to class II GAG peptide and to enhance IL-4-secreting cells against GAG peptides and p24 protein stimuli. Long-lasting humoral and cellular responses were detected until adult age, following neonatal immunization with the chimeric vaccine. The LAMP/gag vaccination was able to induce potent GAG-specific T and B cell immune responses in early life which are essential to elicit sustained and long-lasting mucosal and systemic humoral response.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectDNA vaccines
dc.subjectHIV
dc.subjectMice
dc.subjectMucosal
dc.subjectNeonates
dc.subjectVacinas contra a SIDA / administração & dosagem
dc.subjectVacinas contra a AIDS / genética
dc.subjectVacinas contra a SIDA / imunologia
dc.subjectAnimais
dc.subjectLinfócitos T CD4-positivos / imunologia
dc.subjectLinfócitos T CD8-Positivos / imunologia
dc.subjectCitocinas / imunologia
dc.subjectCitocinas / metabolismo
dc.subjectDependovírus / genética
dc.subjectDependovírus / metabolismo
dc.subjectFêmea
dc.subjectProdutos Genéticos , gag / genética
dc.subjectProdutos Genéticos , gag / immunology
dc.subjectAnticorpos anti- HIV / imunologia
dc.subjectInfecções por HIV / imunologia
dc.subjectImunidade , Mucosa / imunologia
dc.subjectImunização
dc.subjectImunoglobulina A, Secretoria / imunologia
dc.subjectGlicoproteínas de Membrana Associadas a Lisossomo / genética
dc.subjectGlicoproteínas Associadas à Membrana Lisossômica / imunologia
dc.subjectMasculino
dc.subjectRatos
dc.subjectRatos , endogâmicos BALB C
dc.subjectVacinas, DNA / administração & dosagem
dc.subjectVacinas, DNA / genética
dc.subjectVacinas, DNA / imunologia
dc.titleMucosal and systemic anti-GAG immunity induced by neonatal immunization with HIV LAMP/gag DNA vaccine in mice
dc.typeArticle


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