Genetic polymorphism in Leishmania (Viannia) braziliensis detected in mucosal leishmaniasis of HIV-infected and non-HIV-infected patients

dc.creatorOliveira, Fernanda Santos de
dc.creatorValete-Rosalino, Cláudia Maria
dc.creatorSchubach, Armando de Oliveira
dc.creatorMadeira, Maria de Fátima
dc.creatorPacheco, Raquel da Silva
dc.date2017-05-02T11:26:10Z
dc.date2017-05-02T11:26:10Z
dc.date2012
dc.date.accessioned2023-09-26T22:06:06Z
dc.date.available2023-09-26T22:06:06Z
dc.identifierOLIVEIRA, Fernanda Santos de et al. Genetic polymorphism in Leishmania (Viannia) braziliensis detected in mucosal leishmaniasis of HIV-infected and non-HIV-infected patients. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, p. 683- 687, 2012.
dc.identifier0035-9203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18701
dc.identifier10.1016/j.trstmh.2012.07.007
dc.identifier1878-3503
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874745
dc.descriptionThe genetic polymorphism of Leishmania (Viannia) braziliensis detected in cases of mucosal leishmaniasis (ML) from HIV-infected and non HIV-infected patients was evaluated. Nine samples from three HIV-infected patients and five samples from five non HIV-infected patients were analysed by polymerase chain reaction (PCR), low-stringency single-specific primer PCR (LSSP-PCR) and phenetic analysis. The presence of L. (V.) braziliensis DNA was detected in all samples by specific PCR assay. The intraspecific polymorphism of the variable region of L. (V.) braziliensis kDNA minicircles was investigated by LSSP-PCR. Phenetic analysis grouped the genetic profiles into two distinct clusters, which discriminated between samples obtained from HIV-infected and non HIV-infected patients. In two HIV-infected patients, identical genetic profiles were detected in lesions biopsied at different times after the treatment of the initial lesion. Interestingly, genetically divergent profiles were detected in the cutaneous and mucosal lesions of the same HIV-infected patient collected at the same time. This is the first work comparing genetic polymorphism of L. (V.) braziliensis in cases of mucosal leishmaniasis from HIV-infected and non HIV-infected patients.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectLeishmaniose mucosa
dc.subjectLeishmania
dc.subjectHIV
dc.subjectCo-ínfecção
dc.subjectReação em Cadeia da Polimerase
dc.subjectLeishmania braziliensis
dc.subjectMucosal leishmaniasis
dc.subjectLeishmania/HIV co-infection
dc.subjectLeishmania (Viannia) braziliensis
dc.subjectPCR
dc.subjectLSSP-PCR
dc.titleGenetic polymorphism in Leishmania (Viannia) braziliensis detected in mucosal leishmaniasis of HIV-infected and non-HIV-infected patients
dc.typeArticle


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