Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis

dc.creatorRomanos, Maria T. V.
dc.creatorAndrada-Serpa, Maria J.
dc.creatorMourão, Paulo A. S.
dc.creatorYoneshigue-Valentin, Yocie
dc.creatorPereira, Mariana S.
dc.creatorSantos, Norma
dc.creatorWigg, Marcia D.
dc.date2019-11-08T13:18:10Z
dc.date2019-11-08T13:18:10Z
dc.date2011
dc.date.accessioned2023-09-26T22:06:00Z
dc.date.available2023-09-26T22:06:00Z
dc.identifierROMANOS, Maria T. V. et al. Sulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis. Revista Brasileira de Farmacognosia, v. 21, n. 2, p. 229-233, Mar./Apr. 2011.
dc.identifier0102-695X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36954
dc.identifier10.1590/S0102-695X2011005000055
dc.identifier1981-528X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874724
dc.descriptionA sulfated fucan from Laminaria abyssalis marine alga prevented the interaction of HTLV-1 particles, purified from the MT-2 cell line, with HeLa cells. The infection obtained using a concentrated virus suspension was detected only by amplification of the newly synthesized HTLV-1 proviral cDNA by the nestedpolymerase chain reaction (PCR). The sulfated polysaccharide was not toxic to the cells at a concentration of 100 µg/mL and prevented infection by the viral particles when added to the cell monolayers. The proviral cDNA was only detected when the sulfated polysaccharide was added to the cells three hours post-infection, indicating that the inhibitory activity occurred in the initial stages of virus-cell interaction. Our results demonstrate, for the first time, the ability of a sulfated fucan from marine algae to inhibit virus transmission through free virus particles.
dc.description2020-11-08
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectHTLV-1
dc.subjectFree particles
dc.subjectLaminaria abyssalis
dc.subjectMarine algae
dc.subjectSulfated fucan
dc.subjectAntiviral activity
dc.titleSulfated fucan from marine alga inhibits HeLa cells infection by HTLV-1 free particles: semiquantitative analysis
dc.typeArticle


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