Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads

dc.creatorConzentino, Marcelo S.
dc.creatorSantos, Tatielle P.C.
dc.creatorSelim, Khaled A.
dc.creatorWagner, Berenike
dc.creatorAlford, Janette T.
dc.creatorDeobald, Nelli
dc.creatorPaula, Nigela M.
dc.creatorRego, Fabiane G. M.
dc.creatorZanette, Dalila Lucíola
dc.creatorAoki, Mateus Nóbrega
dc.creatorNardin, Jeanine M.
dc.creatorHuergo, Maria C. C.
dc.creatorReis, Rodrigo A.
dc.creatorHuergo, Luciano F.
dc.date2021-10-11T17:39:33Z
dc.date2021-10-11T17:39:33Z
dc.date2021
dc.date.accessioned2023-09-26T22:04:12Z
dc.date.available2023-09-26T22:04:12Z
dc.identifierCONZENTINO, Marcelo S. et al. Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads. Analytical Biochemistry, v.631, n. 114360, p. 1-6, 2021.
dc.identifier0003-2697
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/49337
dc.identifier10.1016/j.ab.2021.114360
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874562
dc.descriptionTo monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsopen access
dc.subjectMagnetic ELISA
dc.subjectSARS-CoV-2
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectMagnetic beads
dc.subjectHigh-Throughput Screening Assays
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectEnsayos Analíticos de Alto Rendimiento
dc.subjectTest ELISA
dc.subjectTests de criblage à haut débit
dc.subjectCOVID-19
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectEnsaios de Triagem em Larga Escala
dc.titleUltra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads
dc.typeArticle


Este ítem pertenece a la siguiente institución