| dc.creator | Vilela, Felipe Pinheiro | |
| dc.creator | Rodrigues, Dália dos Prazeres | |
| dc.creator | Allard, Marc William | |
| dc.creator | Falcão, Juliana Pfrimer | |
| dc.date | 2023-03-05T18:27:53Z | |
| dc.date | 2023-03-05T18:27:53Z | |
| dc.date | 2022 | |
| dc.date.accessioned | 2023-09-26T22:03:37Z | |
| dc.date.available | 2023-09-26T22:03:37Z | |
| dc.identifier | VILELA, Felipe Pinheiro et al. Genomic characterization and antimicrobial resistance profiles of Salmonella enterica serovar Infantis isolated from food, humans and veterinary-related sources in Brazil. Journal of Applied Microbiology, v. 132, p. 3327-3342, 2022. | |
| dc.identifier | 1365-2672 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/57246 | |
| dc.identifier | 10.1111/jam.15430 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8874527 | |
| dc.description | Aims: To characterize the genetic relatedness, phenotypic and genotypic antimicrobial
resistance and plasmid content of 80 Salmonella Infantis strains isolated from
food, humans and veterinary sources from 2013 to 2018 in Brazil.
Methods and results: Pulsed-field
gel electrophoresis and single-nucleotide
polymorphism
analysis showed major clusters containing 50% and 38.8% of the strains
studied respectively. Multilocus sequence typing assigned all strains to ST32. Disk-diffusion
revealed that 90% of the strains presented resistant or intermediate resistant
profiles and 38.8% displayed multidrug resistance. Resistance genes for
aminoglycosides (aac(6′)-Iaa;
aadA12; aph(3″-Ib;
aph(6)-Id),
β-lactams
(blaTEM-1;
blaCTX-M-
8;
blaCMY-2),
trimethoprim (dfrA8), tetracycline (tet(A)), amphenicols (floR),
sulfonamide (sul2), efflux pumps (mdsA; mdsB), chromosomal point mutations in
gyrB, parC, acrB and pmrA were detected. Strains harboured IncI, IncF, IncX, IncQ,
IncN and IncR plasmids.
Conclusions: The presence of a prevalent S. Infantis subtype in Brazil and the high
antimicrobial resistance rates reinforced the potential hazard of this serovar for the
public health and food safety fields.
Significance and Impact of the Study: This is the first study characterizing a large
set of S. Infantis from Brazil by whole-genome
sequencing, which provided a better
local and global comprehension about the distribution and characteristics of this serovar
of importance in the food, human and veterinary fields. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Oxford Academic | |
| dc.rights | open access | |
| dc.subject | Genes de resistência antimicrobiana | |
| dc.subject | Digitação de sequência multilocus | |
| dc.subject | Plasmídeos | |
| dc.subject | Eletroforese em gel de campo pulsado | |
| dc.subject | Salmonella Infantis | |
| dc.subject | Sequenciamento de todo o genoma | |
| dc.subject | Antimicrobial resistance genes | |
| dc.subject | Multilocus sequence typing | |
| dc.subject | Plasmids | |
| dc.subject | Pulsed-field gel electrophoresis | |
| dc.subject | Salmonella Infantis | |
| dc.subject | Whole-genome sequencing | |
| dc.title | Genomic characterization and antimicrobial resistance profiles of Salmonella enterica serovar Infantis isolated from food, humans and veterinary-related sources in Brazil | |
| dc.type | Article | |
