Interferon-Gamma Promotes Infection of Astrocytes by Trypanosoma cruzi.

dc.creatorSilva, Rafael Rodrigues
dc.creatorMeyer, Rafael Mariante
dc.creatorSilva, Andrea Alice
dc.creatorSantos, Ana Luiza Barbosa dos
dc.creatorRoffê, Ester
dc.creatorSantiago, Helton
dc.creatorGazzinelli, Ricardo Tostes
dc.creatorVieira, Joseli Lannes
dc.date2016-01-22T15:27:36Z
dc.date2016-01-22T15:27:36Z
dc.date2015
dc.date.accessioned2023-09-26T22:02:16Z
dc.date.available2023-09-26T22:02:16Z
dc.identifierSILVA, Rafael Rodrigues et al. Interferon-Gamma Promotes Infection of Astrocytes by Trypanosoma cruzi. PLoS One, vol. 10, n. 2, p. e0118600, 2015
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12594
dc.identifier10.1371/journal.pone.0118600. eCollection 2015
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874448
dc.descriptionThe inflammatory cytokine interferon-gamma (IFNγ) is crucial for immunity against intracellular pathogens such as the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease (CD). IFNγ is a pleiotropic cytokine which regulates activation of immune and non-immune cells; however, the effect of IFNγ in the central nervous system (CNS) and astrocytes during CD is unknown. Here we show that parasite persists in the CNS of C3H/He mice chronically infected with the Colombian T. cruzi strain despite the increased expression of IFNγ mRNA. Furthermore, most of the T. cruzi-bearing cells were astrocytes located near IFNγ+ cells. Surprisingly, in vitro experiments revealed that pretreatment with IFNγ promoted the infection of astrocytes by T. cruzi increasing uptake and proliferation of intracellular forms, despite inducing increased production of nitric oxide (NO). Importantly, the effect of IFNγ on T. cruzi uptake and growth is completely blocked by the anti-tumor necrosis factor (TNF) antibody Infliximab and partially blocked by the inhibitor of nitric oxide synthesis L-NAME. These data support that IFNγ fuels astrocyte infection by T. cruzi and critically implicate IFNγ-stimulated T. cruzi-infected astrocytes as sources of TNF and NO, which may contribute to parasite persistence and CNS pathology in CD.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectAstrocytes/drug effects
dc.subjectChagas Disease/immunology
dc.subjectCytokines/metabolism
dc.subjectInterferon-gamma/pharmacology
dc.subjectNG-Nitroarginine Methyl Ester/pharmacology
dc.subjectTrypanosoma cruzi/growth & development
dc.subjectInterferon-gamma
dc.subjectNG-Nitroarginine Methyl Ester
dc.titleInterferon-Gamma Promotes Infection of Astrocytes by Trypanosoma cruzi.
dc.typeArticle


Este ítem pertenece a la siguiente institución