Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection

dc.creatorRodolpho, Joice Margareth de Almeida
dc.creatorCamillo, Luciana
dc.creatorAraújo, Márcio Sobreira Silva
dc.creatorSpeziali, Elaine
dc.creatorReis, Jordana Grazziela Alves Coelho dos
dc.creatorCorreia, Ricardo de Oliveira
dc.creatorNeris, Débora Meira
dc.creatorMartins Filho, Olindo Assis
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorAnibal, Fernanda de Freitas
dc.date2018-09-21T17:54:49Z
dc.date2018-09-21T17:54:49Z
dc.date2018
dc.date.accessioned2023-09-26T22:00:00Z
dc.date.available2023-09-26T22:00:00Z
dc.identifierRODOLPHO, Joice Margareth de Almeida et al. Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection. Frontiers in Immunology, v. 9, p. 1-14, 2018.
dc.identifier1664-3224
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28972
dc.identifier10.3389/fimmu.2018.00064
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874321
dc.descriptionEosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4+ T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role upon Toxocara canis antigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimental T. canis infection. T. canis antigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model for T. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murine T. canis infection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers upon T. canis infection. The enhanced connectivity between eosinophils and T-cells in T. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role during T. canis infection. Moreover, in vitro T. canis antigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimental T. canis infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Research Foundation
dc.rightsopen access
dc.subjectEosinófilos
dc.subjectToxocara canis
dc.subjectSLMV
dc.subjectAtivação
dc.subjectEosinophil
dc.subjectToxocara canis
dc.subjectSLMV
dc.subjectActivation
dc.subjectCo-stimulatory molecules
dc.titleRobust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
dc.typeArticle


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