Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection

dc.creatorKoishi, Andrea Cristine
dc.creatorSuzukawa, Andréia Akemi
dc.creatorZanluca, Camila
dc.creatorCamacho, Daria Elena
dc.creatorComach, Guillermo
dc.creatorSantos, Claudia Nunes Duarte dos
dc.date2019-02-15T14:43:57Z
dc.date2019-02-15T14:43:57Z
dc.date2018
dc.date.accessioned2023-09-26T21:58:08Z
dc.date.available2023-09-26T21:58:08Z
dc.identifierKOISHI, Andrea Cristine et al. Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection. PLoS Negl. Trop. Dis., v. 12, n. 3, p. 1-15, 2018.
dc.identifier1935-2735
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31681
dc.identifier10.1371/journal.pntd.0006342
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8874212
dc.descriptionZika virus (ZIKV) is an emerging arbovirus belonging to the genus flavivirus that comprises other important public health viruses, such as dengue (DENV) and yellow fever (YFV). In general, ZIKV infection is a self-limiting disease, however cases of Guillain-Barré syndrome and congenital brain abnormalities in newborn infants have been reported. Diagnosing ZIKV infection remains a challenge, as viral RNA detection is only applicable until a few days after the onset of symptoms. After that, serological tests must be applied, and, as expected, high cross-reactivity between ZIKV and other flavivirus serology is observed. Plaque reduction neutralization test (PRNT) is indicated to confirm positive samples for being more specific, however it is laborious intensive and time consuming, representing a major bottleneck for patient diagnosis. To overcome this limitation, we developed a high-throughput image-based fluorescent neutralization test for ZIKV infection by serological detection. Using 226 human specimens, we showed that the new test presented higher throughput than traditional PRNT, maintaining the correlation between results. Furthermore, when tested with dengue virus samples, it showed 50.53% less cross reactivity than MAC-ELISA. This fluorescent neutralization test could be used for clinical diagnosis confirmation of ZIKV infection, as well as for vaccine clinical trials and seroprevalence studies.
dc.formatapplication/pdf
dc.languagepor
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectFlavivirus
dc.subjectZika Virus
dc.subjectClinical Diagnosis
dc.subjectSeroepidemiologic Studies
dc.subjectSerologic Tests
dc.subjectVirus Zika
dc.subjectEstudios Seroepidemiológicos
dc.subjectPruebas Serológicas
dc.subjectZika virus
dc.subjectDiagnóstico Clínico
dc.subjectEstudos Soroepidemiológicos
dc.subjectTestes Sorológicos
dc.titleDevelopment and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
dc.typeArticle


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