Illumina sequencing-based analysis of a microbial community enriched under anaerobic methane oxidation condition coupled to denitrification revealed coexistence of aerobic and anaerobic methanotrophs

dc.creatorSiniscalchi, Luciene Alves Batista
dc.creatorLeite, Laura Rabelo
dc.creatorOliveira, Guilherme Correa de
dc.creatorChernicharo, Carlos Augusto Lemos
dc.creatorAraújo, Juliana Calabria de
dc.date2018-05-04T19:43:29Z
dc.date2018-05-04T19:43:29Z
dc.date2017
dc.date.accessioned2023-09-26T21:51:38Z
dc.date.available2023-09-26T21:51:38Z
dc.identifierSINISCALCH, Luciene Alves Batista et al. Illumina sequencing-based analysis of a microbial community enriched under anaerobic methane oxidation condition coupled to denitrification revealed coexistence of aerobic and anaerobic methanotrophs. Environ Sci Pollut Res Int., v. 24, n. 2º, p. 16751-16764, 2017
dc.identifier0944-1344
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26292
dc.identifier10.1007/s11356-017-9197-9
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873845
dc.descriptionMethane is produced in anaerobic environments, such as reactors used to treat wastewaters, and can be consumed by methanotrophs. The composition and structure of a microbial community enriched from anaerobic sewage sludge under methane-oxidation condition coupled to denitrification were investigated. Denaturing gradient gel electrophoresis (DGGE) analysis retrieved sequences of Methylocaldum and Chloroflexi. Deep sequencing analysis revealed a complex community that changed over time and was affected by methane concentration. Methylocaldum (8.2%), Methylosinus (2.3%), Methylomonas (0.02%), Methylacidiphilales (0.45%), Nitrospirales (0.18%), and Methanosarcinales (0.3%) were detected. Despite denitrifying conditions provided, Nitrospirales and Methanosarcinales, known to perform anaerobic methane oxidation coupled to denitrification (DAMO) process, were in very low abundance. Results demonstrated that aerobic and anaerobic methanotrophs coexisted in the reactor together with heterotrophic microorganisms, suggesting that a diverse microbial community was important to sustain methanotrophic activity. The methanogenic sludge was a good inoculum to enrich methanotrophs, and cultivation conditions play a selective role in determining community composition.
dc.description2023-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.rightsrestricted access
dc.subjectprocesso DAMO
dc.subjectDAMO process
dc.subjectIllumina sequencing
dc.subjectMethane
dc.subjectMethanotrophs
dc.subjectSequencing batch reactor
dc.subjectSewage sludge
dc.titleIllumina sequencing-based analysis of a microbial community enriched under anaerobic methane oxidation condition coupled to denitrification revealed coexistence of aerobic and anaerobic methanotrophs
dc.typeArticle


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