Detection of Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in Coahuila state, northern Mexico

dc.creatorOrtega-Morales, Aldo I.
dc.creatorNava-Reyna, Erika
dc.creatorÁvila-Rodríguez, Verónica
dc.creatorGonzález-Álvarez, Vicente H.
dc.creatorCastillo-Martínez, Antonio
dc.creatorSiller-Rodríguez, Quetzaly K.
dc.creatorCabezas-Cruz, Alejandro
dc.creatorDantas-Torres, Filipe
dc.creatorAlmazán, Consuelo
dc.date2019-09-26T13:56:36Z
dc.date2019-09-26T13:56:36Z
dc.date2019
dc.date.accessioned2023-09-26T21:49:37Z
dc.date.available2023-09-26T21:49:37Z
dc.identifierORTEGA-MORALES, Aldo I. et al. Detection of Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in Coahuila state, northern Mexico. Parasites & Vectors, v. 12, n. 1, p. 1-7, Mar. 2019.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35921
dc.identifier10.1186/s13071-019-3377-z
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873730
dc.descriptionBackground: The aim of this study was to detect and molecularly identify Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in 30 communities from fve municipalities in the south of Coahuila State, northern Mexico, where Rocky Mountain spotted fever is endemic. Methods: In total, 60 dogs from each municipality were examined for engorged ticks. DNA was isolated from tick pools and conventional PCR assays targeting the 23S-5S ribosomal RNA intergenic spacer and outer membrane protein (ompA) gene of Rickettsia spp. were performed. Results: All ticks (n = 1238) were morphologically identifed as R. sanguineus (s.l.). Six pools (each with six engorged females) from four municipalities were positive to Rickettsia spp. DNA sequencing and phylogenetic analyses confrmed the presence of R. rickettsii and R. rhipicephali in R. sanguineus (s.l.) in these ticks. Conclusions: This study confrms the presence of R. rickettsii and R. rhipicephali in R. sanguineus (s.l.) from stray dogs in the south of Coahuila. This suggests that stray dogs may play a role in the inter-municipal dissemination of infected ticks in this region. Further research is required to assess whether ticks from stray dogs could serve as good indicators for the molecular xenomonitoring of R. rickettsii in this region. Considering that R. sanguineus (s.l.) is a proven vector of R. rickettsii in Mexico, increased awareness regarding permanent tick control in dogs is warranted.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectCoahuila
dc.subjectMexico
dc.subjectR. rhipicephali
dc.subjectR. rickettsii
dc.subjectR. sanguineus (s.l.)
dc.subjectAnimais
dc.subjectDoenças do Cão
dc.subjectMicrobiologia
dc.subjectDoenças do Cão
dc.subjectParasitologia
dc.subjectCães
dc.subjectFeminino
dc.subjectMasculino
dc.subjectMéxico
dc.subjectTipagem Molecular
dc.subjectVeterinária
dc.subjectFilogenia
dc.subjectReação em Cadeia da Polimerase
dc.subjectRhipicephalus sanguineus
dc.subjectRickettsia
dc.subjectClassificação
dc.subjectIsolamento & purificação
dc.subjectInfestações por Carrapato
dc.titleDetection of Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in Coahuila state, northern Mexico
dc.typeArticle


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