Infection of retinal epithelial cells with L. amazonensis impacts in extracellular matrix proteins

dc.creatorCalabrese, Kátia da Silva
dc.creatorSilva, Leandro de Souza
dc.creatorCarvalho, Luiz Otávio Pereira
dc.creatorHardoim, Daiana de Jesus
dc.creatorAlmeida, Mariana da Silva
dc.creatorMortara, Renato Arruda
dc.creatorSouza, Celeste da Silva Freitas de
dc.date2018-05-10T17:10:34Z
dc.date2018-05-10T17:10:34Z
dc.date2011
dc.date.accessioned2023-09-26T21:47:37Z
dc.date.available2023-09-26T21:47:37Z
dc.identifierCALABRESE, Kátia da Silva; et al. Infection of retinal epithelial cells with L. amazonensis impacts in extracellular matrix proteins. Parasitol Res., v.109, p.727–736, 2011.
dc.identifier0932-0113
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26396
dc.identifier10.1007/s00436-011-2369-5
dc.identifier1432-1955
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873612
dc.descriptionOne of the manifestations of leishmaniases is eye injuries which main characteristics are the injury of the anterior chamber of the eye and the resistance to specific treatments. The retinal pigment epithelial (RPE) cells participate in pathogen-induced intraocular inflammatory processes. We investigated Leishmania amazonensis-RPE cells relationship and its impact in laminin and fibronectin production. Using RPE cell (ARPE-19), we demonstrated that L. amazonensis adhere to these cells in the first hour of infection, whereas parasite internalization was only observed after 6 h. Seventy-two hours after infection, vacuoles with parasites debris were observed intracellularly, and no parasite were observed intra- or extracellularly at the 96 h, suggesting that Leishmania can infect ARPE-19 cells although this cells are able to clear the infection. Fibronectin and laminin were associated with L. amazonensis-ARPE-19 interaction. Confocal analysis showed no substantial alterations in fibronectin presence in ARPE-19-infected or ARPE-19-noninfected cells, whereas laminin levels increased three times 10 h after L. amazonensis infection. After this time, laminin levels decreased in infected cells. These results suggest that L. amazonensis-ARPE-19 infection induces increased production of laminin in the beginning of infection which may facilitate parasite-host cell interactions.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectLeishmania
dc.subjectInfecção
dc.subjectcélulas epiteliais da retina
dc.subjectproteínas da matriz extracelular
dc.subjectL. amazonensis
dc.subjectretinal epithelial cells
dc.subjectinfection
dc.subjectextracellular matrix proteins
dc.titleInfection of retinal epithelial cells with L. amazonensis impacts in extracellular matrix proteins
dc.typeArticle


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