Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves

dc.creatorLeitolis, Amanda
dc.creatorSuss, Paula Hansen
dc.creatorRoderjan, João Gabriel
dc.creatorAngulski, Addeli Bez Batti
dc.creatorCosta, Francisco Diniz Affonso da
dc.creatorStimamiglio, Marco Augusto
dc.creatorDominguez, Alejandro Correa
dc.date2019-05-20T14:02:52Z
dc.date2019-05-20T14:02:52Z
dc.date2019
dc.date.accessioned2023-09-26T21:45:45Z
dc.date.available2023-09-26T21:45:45Z
dc.identifierLEITOLIS, Amanda et al. Human heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves. Int. J. Mol. Sci. , v. 20, n. 1279, p. 1-18, 2019.
dc.identifier1422-0067
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33143
dc.identifier10.3390/ijms20061279
dc.identifier1422-0067
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873506
dc.descriptionExtracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects of EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate human heart-derived extracellular vesicles (hH-EVs). We used nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) to physically characterize hH-EVs and mass spectrometry (MS) to profile the protein content in these particles. The MS analysis identified a total of 1248 proteins. Gene ontology (GO) enrichment analysis in hH-EVs revealed the proteins involved in processes, such as the regulation of cell death and response to wounding. The potential of hH-EVs to induce proliferation, adhesion, angiogenesis and wound healing was investigated in vitro. Our findings demonstrate that hH-EVs have the potential to induce proliferation and angiogenesis in endothelial cells, improve wound healing and reduce mesenchymal stem-cell adhesion. Last, we showed that hH-EVs were able to significantly promote mesenchymal stem-cell recellularization of decellularized porcine heart valve leaflets. Altogether our data confirmed that hH-EVs modulate cellular processes, shedding light on the potential of these particles for tissue regeneration and for scaffold recellularization.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectExplante de Tecido
dc.subjectRecelularização
dc.subjectRegiões Cardíacas
dc.subjectHeart
dc.subjectExtracellular Vesicles
dc.subjectTissue Explant
dc.subjectHeart Valves
dc.subjectTissue Engineering
dc.subjectRecellularization
dc.subjectCardiac Regions
dc.subjectMesenchymal Stromal Cells
dc.subjectCorazón
dc.subjectVálvulas Cardíacas
dc.subjectIngeniería de Tejidos
dc.subjectCélulas del Estroma Mesenquimatoso
dc.subjectCoração
dc.subjectVesículas Extracelulares
dc.subjectValvas Cardíacas
dc.subjectEngenharia Tecidual
dc.subjectCélulas Mesenquimais Estromais
dc.titleHuman heart explant-derived extracellular vesicles: characterization and effects on the in vitro recellularization of decellularized heart valves
dc.typeArticle


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