A comparison of mycolic acid analysis for nontuberculous mycobacteria identification by thin-layer chromatography and molecular methods

dc.creatorLeite, Clarisse Queico Fujimura
dc.creatorRocha, Adalgiza da Silva
dc.creatorLeite, Sergio Roberto de Andrade
dc.creatorFerreira, Rosa Maria Carvalho
dc.creatorSuffys, Philip N.
dc.creatorFonseca, Leila de Souza
dc.creatorSaad, Maria Helena Féres
dc.date2019-12-10T15:18:25Z
dc.date2019-12-10T15:18:25Z
dc.date2005
dc.date.accessioned2023-09-26T21:45:44Z
dc.date.available2023-09-26T21:45:44Z
dc.identifierLEITE, Clarisse Queico Fujimura et al. A Comparison of Mycolic Acid Analysis for Nontuberculous Mycobacteria Identification by Thin-Layer Chromatography and Molecular Methods. Microbiology and Immunology, v. 49, n. 7, p. 571-578, 2005.
dc.identifier0385-5600
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37766
dc.identifier10.1111/j.1348-0421.2005.tb03642.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873505
dc.descriptionThe development of fast, inexpensive, and reliable tests to identify nontuberculous mycobacteria (NTM) is needed. Studies have indicated that the conventional identification procedures, including biochemical assays, are imprecise. This study evaluated a proposed alternative identification method in which 83 NTM isolates, previously identified by conventional biochemical testing and in-house M. avium IS1245-PCR amplification, were submitted to the following tests: thin-layer chromatography (TLC) of mycolic acids and PCR-restriction enzyme analysis of hsp65(PRA). High-performance liquid chromatography (HPLC) analysis of mycolic acids and Southern blot analysis for M. avium IS1245 were performed on the strains that evidenced discrepancies on either of the above tests. Sixty-eight out of 83 (82%) isolates were concordantly identified by the presence of IS1245 and PRA and by TLC mycolic acid analysis. Discrepant results were found between the phenotypic and molecular tests in 12/83 (14.4%) isolates. Most of these strains were isolated from non-sterile body sites and were most probably colonizing in the host tissue. While TLC patterns suggested the presence of polymycobacterial infection in 3/83 (3.6%) cultures, this was the case in only one HPLC-tested culture and in none of those tested by PRA. The results of this study indicated that, as a phenotypic identification procedure, TLC mycolic acid determination could be considered a relatively simple and cost-effective method for routine screening of NTM isolates in mycobacteriology laboratory practice with a potential for use in developing countries. Further positive evidence was that this method demonstrated general agreement on MAC and M. simiae identification, including in the mixed cultures that predominated in the isolates of the disseminated infections in the AIDS patients under study. In view of the fact that the same treatment regimen is recommended for infections caused by these two species, TLC mycolic acid analysis may be a useful identification tool wherever molecular methods are unaffordable.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley 12 Months
dc.rightsrestricted access
dc.subjectÁcidos micólicos
dc.subjectMicobactérias não tuberculosas
dc.subjectMycolic acids
dc.subjectNontuberculous mycobacteria
dc.subjectTLC
dc.subjectIS1245
dc.subjectPRA
dc.titleA comparison of mycolic acid analysis for nontuberculous mycobacteria identification by thin-layer chromatography and molecular methods
dc.typeArticle


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