Contribution of mast cells and snake venom metalloproteinases to the hyperalgesia induced by Bothrops jararaca venom in rats

dc.creatorBonavita, André Gustavo C.
dc.creatorCosta, Aline S. da
dc.creatorPires, Ana Lucia A.
dc.creatorFerreira, Ana G. C. Neves
dc.creatorPerales, Jonas
dc.creatorCordeiro, Renato S. B.
dc.creatorMartins, Marco A.
dc.creatorSilva, Patrícia M. R. e
dc.date2020-01-01T18:56:18Z
dc.date2020-01-01T18:56:18Z
dc.date2006
dc.date.accessioned2023-09-26T21:44:00Z
dc.date.available2023-09-26T21:44:00Z
dc.identifierBONAVITA, André Gustavo C. et al. Contribution of mast cells and snake venom metalloproteinases to the hyperalgesia induced by Bothrops jararaca venom in rats. Toxicon, v. 47, p. 885-893, 2006.
dc.identifier0041-0101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/38927
dc.identifier10.1016/j.toxicon.2006.02.017
dc.identifier1879-3150
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873403
dc.descriptionBothrops jararaca venom (Bjv) is known to induce local inflammation and severe pain. Since, mast cells are able to secrete mediators involved in algesic processes, in this study we examined the putative role of these cells in the hyperalgesia triggered by Bjv in the rat paw. We noted that treatment with mast cell stabilizer sodium cromoglicate as well as with histamine and 5-hydroxytriptamine receptor antagonists meclizine and methysergide, respectively, inhibited the Bjv-induced hyperalgesia. In addition, we showed that stimulation of isolated rat peritoneal mast cells with Bjv in vitro resulted in the release of stored and neo-generated inflammatory mediators such as histamine and leukotriene C(4), respectively. Bjv-induced histamine secretion was clearly sensitive to treatment with sodium cromoglicate and sodium nedocromil. We further observed that metalloproteinase inhibitors 1,10-phenantroline and DM43 inhibited mast cell degranulation in vitro, under conditions where inhibitors of phospholipase A(2) as well as of serine- and cysteine-proteinases were inactive. Altogether, our findings indicate that mast cells seem to contribute to the hyperalgesia caused by Bjv in the rat paw, and also provide evidence that this response might be dependent on the ability of the Bjv to activate directly mast cells.
dc.description2025-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectHiperalgesia
dc.subjectVeneno de jararaca
dc.subjectMastócitos
dc.subjectMetaloproteinase
dc.subjectBothrops jararaca venom
dc.subjectHyperalgesia
dc.subjectMast cells
dc.subjectMetalloproteinase
dc.titleContribution of mast cells and snake venom metalloproteinases to the hyperalgesia induced by Bothrops jararaca venom in rats
dc.typeArticle


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