Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis

dc.creatorCorrêa, José R.
dc.creatorAtella, Georgia C.
dc.creatorBatista, Marcelo M.
dc.creatorSoares, Maurilio J.
dc.date2019-04-04T12:53:25Z
dc.date2019-04-04T12:53:25Z
dc.date2008
dc.date.accessioned2023-09-26T21:39:01Z
dc.date.available2023-09-26T21:39:01Z
dc.identifierCORRÊA, José R. et al. Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis. Experimental Parasitology, v. 119, p. 58-66, Dec. 2008.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32358
dc.identifier10.1016/j.exppara.2007.12.010
dc.identifier1090-2449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873119
dc.descriptionTransferrin uptake by Trypanosoma cruzi epimastigotes occurs mainly through the cytostome/cytopharynx. Here, we present evidences for the association of sterol-rich membrane domains with the transferrin endocytic site. Assays using pharmacological treatments to disrupt clathrin-coated pits and hinder caveolae formation showed no association between transferrin uptake and clathrin-dependent endocytosis, but indicated that cholesterol stability in membrane domains is essential for the endocytosis of transferrin. Furthermore, it was observed a connection between the integrity of cytoskeleton elements at the cytopharynx and the function of the cytostome. Our data show that T. cruzi epimastigotes depend on a specialized pathway for transferrin uptake, which is cholesterol-dependent, clathrin-independent, and closely associated with the structural stability of the cytostome/cytopharynx cytoskeleton.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectMicroscopia eletrônica de transmissão
dc.subjectEndocitose
dc.subjectTransferrina
dc.subjectCitoesqueleto
dc.subjectCitostome
dc.subjectColesterol da membrana
dc.subjectTrypanosoma cruzi
dc.subjectTransmission electron microscopy
dc.subjectTransferrin
dc.subjectEndocytosis
dc.subjectCytoskeleton
dc.subjectMembrane cholesterol
dc.subjectClathrin
dc.titleTransferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis
dc.typeArticle


Este ítem pertenece a la siguiente institución