Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.

dc.creatorFerraro, Geraldo Argolo
dc.creatorMello, Marco Antônio Gomes
dc.creatorSutmöller, Frits
dc.creatorVan Weyenbergh, Johan Jozef Rosa Maria
dc.creatorBrazilian Network For HIV isolation and characterizationI
dc.creatorShindo, Nice
dc.creatorCastro Filho, Bernardo Galvão
dc.creatorBou-Habib, Dumith Chequer
dc.date2014-08-06T19:04:13Z
dc.date2014-08-06T19:04:13Z
dc.date2001
dc.date.accessioned2023-09-26T21:37:47Z
dc.date.available2023-09-26T21:37:47Z
dc.identifierFERRARO, G. A. et al. Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil. AIDS Research and Human Retroviruses, v. 17, n. 13, p. 1241-1247, 2001.
dc.identifier0889-2229
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8146
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8873055
dc.descriptionThe human immunodeficiency virus type 1 (HIV-1), the etiological agent of the acquired immunodeficiency syndrome (AIDS), shows a variety of biological properties, which may constitute an obstacle to development of effective vaccines or antiretroviral therapy. To characterize Brazilian strains of HIV-1, we studied 24 viruses isolated from blood samples of HIV-1-positive patients from different regions of the country. To examine the cell tropism and the virus ability to form syncytia, primary macrophages and the CD41 T cell line MT-2 were infected with these viruses. We found that 22 isolates replicated well in macrophages (macrophage-tropic isolates), 2 infected only MT-2 cells (T cell line tropic variants), while 6 of them grew in both cells. We found 8 syncytium-inducing (SI) and 16 non-SI (NSI) isolates. Continuous cultures of 18 isolates were established in the CCR51 /CXCR41 cell line PM-1, and SI/NSI features of these viruses were confirmed by cell fusion assay with uninfected CD41 T cell lines (PM-1, MT-2, H9, and SUP-T1). The coreceptor usage of 18 isolates was investigated by infecting U87 cells transfected with CD4 and chemokine receptors, and we found that 11 isolates infected only CCR51 cells, 3 only CXCR41 cells, whereas 4 used both coreceptors. We also observed that X4 isolates were more sensitive to neutralization by dextran sulfate than R5 or R5X4 viruses. Our findings show that the Brazilian isolates are phenotypically similar to those prevalent in other regions, which could mean that therapeutic strategies based on HIV-1 phenotypic properties would be efficient in Brazil, as in other countries.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMary Ann Liebert, Inc
dc.rightsopen access
dc.subjectInfecções por HIV/virologia
dc.subjectHIV-1/isolamento & purificação
dc.subjectHIV-1/metabolismo
dc.subjectReceptores CCR5/metabolismo
dc.subjectBrasil/epidemiologia
dc.subjectLinhagem Celular
dc.subjectSulfato de Dextrana/farmacologia
dc.subjectCélulas Gigantes/efeitos de drogas
dc.subjectInfecções por HIV/epidemiologia
dc.subjectHIV-1/efeitos de drogas
dc.subjectHIV-1/genética
dc.subjectHumanos
dc.subjectMacrófagos/efeitos de drogas
dc.subjectMacrófagos/virologia
dc.subjectEspecificidade por Substrato
dc.titleBiological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
dc.typeArticle


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