Early detection and persistent positivity of anti‑Leishmania antibodies using a recombinant protein‑based ELISA in naturally infected dogs in Brazil

dc.creatorJesus, Matheus Silva de
dc.creatorCruz, João Victor Andrade
dc.creatorCoelho, Lívia Brito
dc.creatorBorja, Lairton Souza
dc.creatorSilva, Edmilson Domingos da
dc.creatorBrodskyn, Claudia Ida
dc.creatorSolcà, Manuela da Silva
dc.creatorFraga, Deborah Bittencourt Mothé
dc.date2021-09-20T11:57:00Z
dc.date2021-09-20T11:57:00Z
dc.date2021
dc.date.accessioned2023-09-26T21:35:47Z
dc.date.available2023-09-26T21:35:47Z
dc.identifierJESUS, Matheus Silva de et al. Early detection and persistent positivity of anti‑Leishmania antibodies using a recombinant protein‑based ELISA in naturally infected dogs in Brazil. Parasites and Vectors, 2021.
dc.identifier1756-3305
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/49114
dc.identifier10.1186/s13071-021-04895-z
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8872938
dc.descriptionFoundation for Research Support of the State of Bahia (Grants JCB0010/2013, SUS0036/2013 and PET0024/2013) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.
dc.descriptionVisceral leishmaniasis (VL) is a zoonotic disease caused by Leishmania infantum, for which dogs constitute the main urban parasite reservoir. Control measures and the treatment of canine visceral leishmaniasis (CVL) are essential to reduce VL cases. Early and accurate detection of L. infantum-infected dogs is crucial to the success of VL control. To improve the serological detection of L. infantum-exposed dogs, we evaluated the early diagnosis capacity of a recombinant protein (rLci5) in an immunosorbent assay (ELISA) to detect naturally infected dogs. Additionally, we evaluated the persistence of the positive results obtained by rLci5 ELISA in comparison to other conventional diagnostic test methods. Methods: Serum samples obtained from 48 L. infantum-infected dogs involved in a cohort study were evaluated using different diagnostic methods (qPCR, EIE-LVC, DPP-LVC and splenic culture). The results were compared to rLci5 ELISA to determine its capacity to diagnose L. infantum infection at earlier infection time points. The persistence of positive diagnostic test results was also compared for each dog evaluated. Results: rLci5 ELISA presented higher rates of positive results at early time points compared to the other diagnostic tests employed in the cohort study, as early as 24 months prior to detection by other tests. rLci5 ELISA positivity was 52.1% (25/48) at baseline, while qPCR was 35.4% (17/48), DPP-LVC 27.1% (13/48), EIE-LVC 22.9% (11/48) and culture only 4.2% (2/48). In at least one of the time points of the 24-month cohort study, rLci5 ELISA was positive in 100% (48/48) of the dogs, versus 83% (40/48) for qPCR, 75% (36/48) for DPP-LVC, 65% (31/48) for EIE-LVC and 31% (15/48) for culture. Investigating clinical signs in association with diagnostic test positivity, rLci5 ELISA successfully detected CVL in 62.9% (95/151) of the clinical evaluations with a score of 0–3, 64.3% (45/70) with scores between 4 and 7, and 73.7% (14/19) with scores > 7, providing higher rates of positivity than all other methods evaluated. Moreover, rLci5 ELISA presented the greatest persistence with respect to test positivity: 45.8% of the dogs evaluated. Conclusion: Four diagnostic tests were compared to rLci5 ELISA, which presented earlier infection diagnosis and a greater persistence of positive test results performance by detecting infected dogs sooner than other testing methods, with enhanced persistence of positive results over the course of the infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBMC
dc.rightsopen access
dc.subjectDiagnóstico precoce
dc.subjectLeishmania
dc.subjectLeishmania infantum
dc.subjectAnticorpos
dc.subjectEarly diagnosis
dc.subjectCanine leishmaniasis
dc.subjectrLci5
dc.subjectDiagnostic persistence
dc.titleEarly detection and persistent positivity of anti‑Leishmania antibodies using a recombinant protein‑based ELISA in naturally infected dogs in Brazil
dc.typeArticle


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